PKM sirna

PKM siRNA (Mouse)

Cat. Num. AAA8236133

• Gene Names: Pkm; Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PKM; PKM siRNA (Mouse); PK3; PKM2; PYKM; Pyruvate kinase PKM; Pyruvate kinase muscle isozyme; PKM sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PKM siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 531

• Applicable Applications for PKM sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PKM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PKM sirna

siRNA to inhibit PKM expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 359807367
• NCBI GeneID: 18746
• NCBI Accession #: NP_001240812.1
• NCBI GenBank Nucleotide #: NM_001253883.1
• UniProt Accession #: P52480; Q3TBV8; Q3TBW5; Q3TC59; Q3U1X3; Q3U5P6; Q4VC20; Q64484; Q91YI8; Q9CWB1
• Molecular Weight: 57,985 Da
• NCBI Official Full Name: pyruvate kinase PKM isoform M1
• NCBI Official Synonym Full Names: pyruvate kinase, muscle
• NCBI Official Symbol: Pkm
• NCBI Official Synonym Symbols: Pk3; Pk-2; Pk-3; Pkm2; AA414905; AL024370; AL024424
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• Protein Family: Pyruvate kinase
• UniProt Gene Name: Pkm
• UniProt Synonym Gene Names: Pk3; Pkm2; Pykm
• UniProt Entry Name: KPYM_MOUSE

Uniprot Description

PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.

Protein type: Mitochondrial; Nucleotide Metabolism - purine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Kinase, other

Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; vesicle; myelin sheath; cilium

Molecular Function: transferase activity; potassium ion binding; protein binding; metal ion binding; magnesium ion binding; nucleotide binding; kinase activity; ADP binding; pyruvate kinase activity; catalytic activity; ATP binding

Biological Process: pyruvate biosynthetic process; organ regeneration; glycolysis; metabolic process; ATP biosynthetic process; programmed cell death; glucose metabolic process; skeletal muscle regeneration; liver development; phosphorylation