SPG11 sirna

SPG11 siRNA (Human)

Cat. Num. AAA8236080

• Gene Names: SPG11; KIAA1840
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPG11; SPG11 siRNA (Human); KIAA1840; Spatacsin; Colorectal carcinoma-associated protein; Spastic paraplegia 11 protein; SPG11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPG11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,330

• Applicable Applications for SPG11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPG11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPG11 sirna

siRNA to inhibit SPG11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 237681069
• NCBI GeneID: 80208
• NCBI Accession #: NP_001153699.1
• NCBI GenBank Nucleotide #: NM_001160227.1
• UniProt Accession #: Q96JI7; Q4VC11; Q58G86; Q69YG6; Q6NW01; Q8N270; Q8TBU9; Q9H734; A8KAX9; B9EK60; F5H3N6
• Molecular Weight: 266,643 Da
• NCBI Official Full Name: spatacsin isoform 2
• NCBI Official Synonym Full Names: spastic paraplegia 11 (autosomal recessive)
• NCBI Official Symbol: SPG11
• NCBI Official Synonym Symbols: KIAA1840
• NCBI Protein Information: spatacsin
• UniProt Protein Name: Spatacsin
• Protein Family: Spatacsin
• UniProt Gene Name: SPG11
• UniProt Synonym Gene Names: KIAA1840
• UniProt Entry Name: SPTCS_HUMAN

NCBI Description

The protein encoded by this gene is a potential transmembrane protein that is phosphorylated upon DNA damage. Defects in this gene are a cause of spastic paraplegia type 11 (SPG11). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

SPG11: Defects in SPG11 are the cause of spastic paraplegia autosomal recessive type 11 (SPG11). Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: lysosomal membrane; cytoplasm; integral to membrane; plasma membrane; nucleolus; cytoplasmic vesicle; cytosol

Molecular Function: protein binding

Disease: Spastic Paraplegia 11, Autosomal Recessive