HLA-DOB sirna

HLA-DOB siRNA (Human)

Cat. Num. AAA8235251

• Gene Names: HLA-DOB; DOB
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HLA-DOB; HLA-DOB siRNA (Human); HLA class II histocompatibility antigen DO beta chain; MHC class II antigen DOB; HLA-DOB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HLA-DOB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 273

• Applicable Applications for HLA-DOB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HLA-DOB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HLA-DOB sirna

siRNA to inhibit HLA-DOB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4504403
• NCBI GeneID: 3112
• NCBI Accession #: NP_002111.1
• NCBI GenBank Nucleotide #: NM_002120.3
• UniProt Accession #: P13765; Q29746; Q29825; Q6FHC2; B0V0Y0
• Molecular Weight: 30,822 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen, DO beta chain
• NCBI Official Synonym Full Names: major histocompatibility complex, class II, DO beta
• NCBI Official Symbol: HLA-DOB
• NCBI Official Synonym Symbols: DOB
• NCBI Protein Information: HLA class II histocompatibility antigen, DO beta chain
• UniProt Protein Name: HLA class II histocompatibility antigen, DO beta chain
• Protein Family: HLA class II histocompatibility antigen
• UniProt Gene Name: HLA-DOB
• UniProt Entry Name: DOB_HUMAN

NCBI Description

HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-DOB: Important modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B-cells. Modifies peptide exchange activity of HLA-DM. Belongs to the MHC class II family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: lysosome; lysosomal membrane; integral to membrane; endosome membrane; MHC class II protein complex

Molecular Function: MHC class II receptor activity

Biological Process: negative regulation of antigen processing and presentation of peptide antigen via MHC class II; antigen processing and presentation of exogenous peptide antigen via MHC class II; immune response; signal transduction