IL4I1 sirna

IL4I1 siRNA (Human)

Cat. Num. AAA8235087

• Gene Names: IL4I1; LAO; FIG1; LAAO
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IL4I1; IL4I1 siRNA (Human); FIG1; L-amino-acid oxidase; LAAO; LAO; Interleukin-4-induced protein 1; IL4-induced protein 1; Protein Fig-1; hFIG1; IL4I1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: IL4I1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 589

• Applicable Applications for IL4I1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human IL4I1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IL4I1 sirna

siRNA to inhibit IL4I1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 384381475
• NCBI GeneID: 259307
• NCBI Accession #: NP_001244946.1
• NCBI GenBank Nucleotide #: NM_001258017.1
• UniProt Accession #: Q96RQ9; Q1WMJ3; Q4GZN1; Q4GZN2; Q6P2Q3; Q8TEM5; Q96RQ8
• Molecular Weight: 65,328 Da
• NCBI Official Full Name: L-amino-acid oxidase isoform 2
• NCBI Official Synonym Full Names: interleukin 4 induced 1
• NCBI Official Symbol: IL4I1
• NCBI Official Synonym Symbols: LAO; FIG1; LAAO
• NCBI Protein Information: L-amino-acid oxidase
• UniProt Protein Name: L-amino-acid oxidase
• UniProt Gene Name: IL4I1
• UniProt Synonym Gene Names: FIG1; LAAO; LAO; IL4-induced protein 1; hFIG1
• UniProt Entry Name: OXLA_HUMAN

NCBI Description

This gene encodes a protein with limited similarity to L-amino acid oxidase which contains the conserved amino acids thought to be involved in catalysis and binding of flavin adenine dinucleotide (FAD) cofactor. The expression of this gene can be induced by interleukin 4 in B cells, however, expression of transcripts containing the first two exons of the upstream gene is found in other cell types. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

IL4I1: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation. Belongs to the flavin monoamine oxidase family. FIG1 subfamily. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; EC 1.4.3.2; Amino Acid Metabolism - cysteine and methionine; Secreted, signal peptide; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Oxidoreductase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - phenylalanine; Secreted

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: lysosome; extracellular region

Molecular Function: L-amino-acid oxidase activity