PINX1 sirna

PINX1 siRNA (Mouse)

Cat. Num. AAA8234869

• Gene Names: Pinx1; LPTS; LPTS1; 67-11-3; AU024023; 2210403I16Rik; 2610028A01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PINX1; PINX1 siRNA (Mouse); LPTS; PIN2/TERF1-interacting telomerase inhibitor 1; 67-11-3 protein; LPTS1; Liver-related putative tumor suppressor; Pin2-interacting protein X1; TRF1-interacting protein 1; PINX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PINX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 332

• Applicable Applications for PINX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PINX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PINX1 sirna

siRNA to inhibit PINX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 18129616
• NCBI GeneID: 72400
• NCBI Accession #: NP_082504.1
• NCBI GenBank Nucleotide #: NM_028228.3
• UniProt Accession #: Q9CZX5; Q14BS4; Q3V450; Q8C6E5; Q91WZ9; Q9D0C2
• Molecular Weight: 37,221 Da
• NCBI Official Full Name: PIN2/TERF1-interacting telomerase inhibitor 1
• NCBI Official Synonym Full Names: PIN2/TERF1 interacting, telomerase inhibitor 1
• NCBI Official Symbol: Pinx1
• NCBI Official Synonym Symbols: LPTS; LPTS1; 67-11-3; AU024023; 2210403I16Rik; 2610028A01Rik
• NCBI Protein Information: PIN2/TERF1-interacting telomerase inhibitor 1
• UniProt Protein Name: PIN2/TERF1-interacting telomerase inhibitor 1
• Protein Family: PIN2/TERF1-interacting telomerase inhibitor
• UniProt Gene Name: Pinx1
• UniProt Synonym Gene Names: Lpts
• UniProt Entry Name: PINX1_MOUSE

Uniprot Description

PINX1: Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor. Belongs to the PINX1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Tumor suppressor; Inhibitor

Cellular Component: kinetochore; nucleoplasm; nuclear chromosome; chromosome, telomeric region; intracellular membrane-bound organelle; mitochondrion; cytoplasm; nucleolus; spindle; chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein binding; nucleic acid binding

Biological Process: telomere maintenance via telomerase; mitotic metaphase plate congression