CREB3L1 sirna

CREB3L1 siRNA (Human)

Cat. Num. AAA8232880

• Gene Names: CREB3L1; OASIS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CREB3L1; CREB3L1 siRNA (Human); OASIS; Cyclic AMP-responsive element-binding protein 3-like protein 1; cAMP-responsive element-binding protein 3-like protein 1; Old astrocyte specifically-induced substance; CREB3L1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CREB3L1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 519

• Applicable Applications for CREB3L1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CREB3L1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CREB3L1 sirna

siRNA to inhibit CREB3L1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16418355
• NCBI GeneID: 90993
• NCBI Accession #: NP_443086.1
• NCBI GenBank Nucleotide #: NM_052854.3
• UniProt Accession #: Q96BA8; Q8N2D5; Q96CP0
• Molecular Weight: 47,783 Da
• NCBI Official Full Name: cyclic AMP-responsive element-binding protein 3-like protein 1
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 3-like 1
• NCBI Official Symbol: CREB3L1
• NCBI Official Synonym Symbols: OASIS
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 3-like protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 3-like protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein 3-like protein
• UniProt Gene Name: CREB3L1
• UniProt Synonym Gene Names: OASIS; cAMP-responsive element-binding protein 3-like protein 1; OASIS
• UniProt Entry Name: CR3L1_HUMAN

NCBI Description

The protein encoded by this gene is normally found in the membrane of the endoplasmic reticulum (ER). However, upon stress to the ER, the encoded protein is cleaved and the released cytoplasmic transcription factor domain translocates to the nucleus. There it activates the transcription of target genes by binding to box-B elements. [provided by RefSeq, Jun 2013]

Uniprot Description

CREB3L1: Transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE. Belongs to the bZIP family. ATF subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane; nucleus

Molecular Function: sequence-specific DNA binding; chromatin binding

Biological Process: transcription, DNA-dependent; unfolded protein response; regulation of bone mineralization