DUSP14 sirna

DUSP14 siRNA (Human)

Cat. Num. AAA8232390

• Gene Names: DUSP14; MKP6; MKP-L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DUSP14; DUSP14 siRNA (Human); MKP6; Dual specificity protein phosphatase 14; MKP-1-like protein tyrosine phosphatase; MKP-L; Mitogen-activated protein kinase phosphatase 6; MAP kinase phosphatase 6; MKP-6; DUSP14 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: DUSP14 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 198

• Applicable Applications for DUSP14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human DUSP14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DUSP14 sirna

siRNA to inhibit DUSP14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5902002
• NCBI GeneID: 11072
• NCBI Accession #: NP_008957.1
• NCBI GenBank Nucleotide #: NM_007026.3
• UniProt Accession #: O95147
• Molecular Weight: 22,255 Da
• NCBI Official Full Name: dual specificity protein phosphatase 14
• NCBI Official Synonym Full Names: dual specificity phosphatase 14
• NCBI Official Symbol: DUSP14
• NCBI Official Synonym Symbols: MKP6; MKP-L
• NCBI Protein Information: dual specificity protein phosphatase 14
• UniProt Protein Name: Dual specificity protein phosphatase 14
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP14
• UniProt Synonym Gene Names: MKP6; MKP-L; MAP kinase phosphatase 6; MKP-6
• UniProt Entry Name: DUS14_HUMAN

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]

Uniprot Description

MKP-6: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). Is a negative regulator of T cell costimulation via CD28.

Protein type: EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 17q12

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: inactivation of MAPK activity