TBCE sirna

TBCE siRNA (Human)

Cat. Num. AAA8232248

• Gene Names: TBCE; HRD; KCS; KCS1; pac2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TBCE; TBCE siRNA (Human); Tubulin-specific chaperone E; Tubulin-folding cofactor E; TBCE sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: TBCE siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 527

• Applicable Applications for TBCE sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human TBCE gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TBCE sirna

siRNA to inhibit TBCE expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 118442828
• NCBI GeneID: 6905
• NCBI Accession #: NP_001072983.1
• NCBI GenBank Nucleotide #: NM_001079515.2
• UniProt Accession #: Q15813; A8K8C2; B7Z3P1
• Molecular Weight: 64,852 Da
• NCBI Official Full Name: tubulin-specific chaperone E isoform a
• NCBI Official Synonym Full Names: tubulin folding cofactor E
• NCBI Official Symbol: TBCE
• NCBI Official Synonym Symbols: HRD; KCS; KCS1; pac2
• NCBI Protein Information: tubulin-specific chaperone E
• UniProt Protein Name: Tubulin-specific chaperone E
• Protein Family: Tubulin-specific chaperone
• UniProt Gene Name: TBCE
• UniProt Entry Name: TBCE_HUMAN

NCBI Description

Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TBCE: Tubulin-folding protein; involved in the second step of the tubulin folding pathway. Seems to be implicated in the maintenance of the neuronal microtubule network. Involved in regulation of tubulin heterodimer dissociation. Defects in TBCE are a cause of hypoparathyroidism- retardation-dysmorphism syndrome (HRD); also known as hypoparathyroidism with short stature, mental retardation, and seizures or Sanjad-Sakati syndrome. HRD is an autosomal recessive disorder reported almost exclusively in Middle Eastern populations. Defects in TBCE are the cause of Kenny-Caffey syndrome type 1 (KCS1). KCS1 is similar to HRD with the additional features of osteosclerosis and recurrent bacterial infections. Belongs to the TBCE family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: microtubule; cytoplasm

Molecular Function: chaperone binding

Biological Process: developmental growth; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; adult locomotory behavior; muscle atrophy; microtubule cytoskeleton organization and biogenesis; post-chaperonin tubulin folding pathway; peripheral nervous system neuron axonogenesis; post-embryonic development

Disease: Hypoparathyroidism-retardation-dysmorphism Syndrome; Kenny-caffey Syndrome, Type 1