ATP1A1 sirna

ATP1A1 siRNA (Rat)

Cat. Num. AAA8231335

• Gene Names: Atp1a1; Nkaa1b
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP1A1; ATP1A1 siRNA (Rat); Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1; ATP1A1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ATP1A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1023

• Applicable Applications for ATP1A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ATP1A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP1A1 sirna

siRNA to inhibit ATP1A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978543
• NCBI GeneID: 24211
• NCBI Accession #: NP_036636.1
• NCBI GenBank Nucleotide #: NM_012504.1
• UniProt Accession #: P06685; Q64609
• Molecular Weight: 113,054 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-1
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• NCBI Official Symbol: Atp1a1
• NCBI Official Synonym Symbols: Nkaa1b
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-1
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: Atp1a1
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-1 subunit
• UniProt Entry Name: AT1A1_RAT

NCBI Description

mediates Na+ and K+ transport; may play a role in regulation of blood pressure [RGD, Feb 2006]

Uniprot Description

ATP1A1: the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described.

Protein type: Transporter; Membrane protein, multi-pass; EC 3.6.3.9; Hydrolase; Transporter, ion channel; Membrane protein, integral

Cellular Component: Golgi apparatus; protein complex; endoplasmic reticulum; basolateral plasma membrane; T-tubule; integral to membrane; caveola; lipid raft; membrane; apical plasma membrane; plasma membrane; melanosome; sodium:potassium-exchanging ATPase complex; endosome; myelin sheath; vesicle; sarcolemma

Molecular Function: potassium ion binding; protein domain specific binding; protein binding; sodium ion binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; negative regulation of heart contraction; membrane hyperpolarization; sodium ion transport; positive regulation of striated muscle contraction; regulation of sodium ion transport; cellular potassium ion homeostasis; negative regulation of glucocorticoid biosynthetic process; potassium ion import; dephosphorylation; energy coupled proton transport, against electrochemical gradient; regulation of blood pressure; positive regulation of heart contraction; potassium ion transport; regulation of the force of heart contraction; cardiac muscle contraction