RP9 sirna

RP9 siRNA (Human)

Cat. Num. AAA8230967

• Gene Names: RP9; PAP1; PAP-1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RP9; RP9 siRNA (Human); Retinitis pigmentosa 9 protein; Pim-1-associated protein; PAP-1; RP9 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RP9 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 221

• Applicable Applications for RP9 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RP9 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RP9 sirna

siRNA to inhibit RP9 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42718020
• NCBI GeneID: 6100
• NCBI Accession #: NP_976033.1
• NCBI GenBank Nucleotide #: NM_203288.1
• UniProt Accession #: Q8TA86
• Molecular Weight: 26,107 Da
• NCBI Official Full Name: retinitis pigmentosa 9 protein
• NCBI Official Synonym Full Names: retinitis pigmentosa 9 (autosomal dominant)
• NCBI Official Symbol: RP9
• NCBI Official Synonym Symbols: PAP1; PAP-1
• NCBI Protein Information: retinitis pigmentosa 9 protein
• UniProt Protein Name: Retinitis pigmentosa 9 protein
• Protein Family: Retinitis pigmentosa 9 protein
• UniProt Gene Name: RP9
• UniProt Synonym Gene Names: PAP-1
• UniProt Entry Name: RP9_HUMAN

NCBI Description

The protein encoded by this gene can be bound and phosphorylated by the protooncogene PIM1 product, a serine/threonine protein kinase . This protein localizes in nuclear speckles containing the splicing factors, and has a role in pre-mRNA splicing. CBF1-interacting protein (CIR), a corepressor of CBF1, can also bind to this protein and effects alternative splicing. Mutations in this gene result in autosomal dominant retinitis pigmentosa-9. This gene has a pseudogene (GeneID: 441212), which is located in tandem array approximately 166 kb distal to this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

RP9: Is thought to be a target protein for the PIM1 kinase. May play some roles in B-cell proliferation in association with PIM1. Defects in RP9 are the cause of retinitis pigmentosa type 9 (RP9). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP9 inheritance is autosomal dominant.

Protein type: RNA processing

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: nucleoplasm; cytoplasm; signal recognition particle receptor complex; nucleus

Molecular Function: protein binding; zinc ion binding

Biological Process: RNA splicing; cognition

Disease: Retinitis Pigmentosa 9