POLR2I blocking peptide

POLR2I Blocking Peptide

Cat. Num. AAA823096

• Gene Names: POLR2I; RPB9; hRPB14.5
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig, Rabbit
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: POLR2I; POLR2I Blocking Peptide; DNA-directed RNA polymerase II subunit RPB9; RNA polymerase II subunit B9; DNA-directed RNA polymerase II subunit I; RNA polymerase II 14.5 kDa subunit; RPB14.5; POLR2I blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig, Rabbit
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 125

• Applicable Applications for POLR2I blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLR2I blocking peptide

The peptide is used to block Anti-POLR2I Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 5453930
• NCBI GeneID: 5438
• NCBI Accession #: NP_006224.1
• NCBI GenBank Nucleotide #: NM_006233.4
• UniProt Accession #: P36954; Q6NW05; B2R5J2
• Molecular Weight: 14,523 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB9
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• NCBI Official Symbol: POLR2I
• NCBI Official Synonym Symbols: RPB9; hRPB14.5
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB9; RPB14.5; RNA polymerase II subunit B9; RNA polymerase II 14.5 kDa subunit; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II 14.5 kDa polypeptide
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB9
• UniProt Gene Name: POLR2I
• UniProt Synonym Gene Names: RNA polymerase II subunit B9; RPB14.5
• UniProt Entry Name: RPB9_HUMAN

NCBI Description

This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2I: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template. Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.

Protein type: RNA processing; Nucleotide Metabolism - purine; Transferase; EC 2.7.7.6; Nucleotide Metabolism - pyrimidine; Transcription initiation complex

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleoplasm; DNA-directed RNA polymerase II, core complex; nucleus

Molecular Function: DNA binding; zinc ion binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; transcription initiation from RNA polymerase II promoter; mRNA capping; viral reproduction; somatic stem cell maintenance; nucleotide-excision repair; positive regulation of viral transcription; transcription-coupled nucleotide-excision repair; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression; DNA repair