Rabbit anti-Mouse, Rat KC Polyclonal Antibody | anti-KC antibody

KC Antibody

Cat. Num. AAA840325

• Gene Names: Cxcl1; Gro1; CINC-1
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Neutralization Assay
• Purity: Affinity purified
• Synonyms: KC; Polyclonal Antibody; KC Antibody; GRO alpha; GRO; chemokine (C-X-C); anti-KC antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) protein A purified rabbit anti-KC polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 96

• Applicable Applications for anti-KC antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Neutralization
• Application Notes: Western blot analysis (0.5-4 ug/ml), Immunoprecipitation (3-5 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
• Immunogen: E. coli-expressed rat KC
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-KC antibody

Background: KC, a homolog of human and hamster gro/MGSA, is a 72-amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of netrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1beta stimulation. IFN-g blocks LPS-induced expression of KC.

NCBI and Uniprot Product Information

• NCBI GI #: 206687
• NCBI GeneID: 81503
• Molecular Weight: 10,249 Da
• NCBI Official Full Name: KC protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: Gro1; CINC-1
• NCBI Protein Information: growth-regulated alpha protein
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: KCNQ1 downstream neighbor protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Cinc1; Gro; Scyb1; CINC-1
• UniProt Entry Name: GROA_RAT

NCBI Description

acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of potassium ion transport; response to glucocorticoid stimulus; response to amphetamine; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; response to molecule of bacterial origin; positive regulation of superoxide release; response to gamma radiation; immune response; acute inflammatory response