COPS5 sirna

COPS5 siRNA (Mouse)

Cat. Num. AAA8229731

• Gene Names: Cops5; CSN5; Jab1; Sgn5; Mov34; AI303502
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COPS5; COPS5 siRNA (Mouse); CSN5; JAB1; KIC2; COP9 signalosome complex subunit 5; SGN5; Signalosome subunit 5; Jun activation domain-binding protein 1; Kip1 C-terminus-interacting protein 2; COPS5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COPS5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for COPS5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COPS5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COPS5 sirna

siRNA to inhibit COPS5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 459447369
• NCBI GeneID: 26754
• NCBI Accession #: NP_001264030.1
• NCBI GenBank Nucleotide #: NM_001277101.1
• UniProt Accession #: O35864; Q3UA70; Q8C1S1
• Molecular Weight: 37,549 Da
• NCBI Official Full Name: COP9 signalosome complex subunit 5 isoform 2
• NCBI Official Synonym Full Names: COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana)
• NCBI Official Symbol: Cops5
• NCBI Official Synonym Symbols: CSN5; Jab1; Sgn5; Mov34; AI303502
• NCBI Protein Information: COP9 signalosome complex subunit 5
• UniProt Protein Name: COP9 signalosome complex subunit 5
• UniProt Gene Name: Cops5
• UniProt Synonym Gene Names: Csn5; Jab1; Kic2; SGN5; Signalosome subunit 5
• UniProt Entry Name: CSN5_MOUSE

Uniprot Description

COPS5: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B). The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, p27Kip1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Belongs to the peptidase M67A family. CSN5 subfamily.

Protein type: Nuclear receptor co-regulator; EC 3.4.-.-; Translation; Protease; Transcription, coactivator/corepressor; Adaptor/scaffold

Cellular Component: nucleoplasm; signalosome; synaptic vesicle; transcription factor complex; cytoplasm; synapse; cytoplasmic vesicle; cell junction; nucleus

Molecular Function: peptidase activity; protein binding; metallopeptidase activity; hydrolase activity; metal ion binding; transcription coactivator activity; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: protein deneddylation; cullin deneddylation; protein deubiquitination; regulation of transcription, DNA-dependent; regulation of cell cycle; positive regulation of transcription from RNA polymerase II promoter; proteolysis; regulation of JNK cascade