CYTH2 sirna

CYTH2 siRNA (Human)

Cat. Num. AAA8229661

• Gene Names: CYTH2; ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYTH2; CYTH2 siRNA (Human); ARNO; PSCD2; PSCD2L; Cytohesin-2; ARF exchange factor; ARF nucleotide-binding site opener; Protein ARNO; PH. SEC7 and coiled-coil domain-containing protein 2; CYTH2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CYTH2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 399

• Applicable Applications for CYTH2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CYTH2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYTH2 sirna

siRNA to inhibit CYTH2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 195972859
• NCBI GeneID: 9266
• NCBI Accession #: NP_004219.3
• NCBI GenBank Nucleotide #: NM_004228.6
• UniProt Accession #: Q99418; Q8IXY9; Q92958; A8K8P0
• Molecular Weight: 46,489 Da
• NCBI Official Full Name: cytohesin-2 isoform 2
• NCBI Official Synonym Full Names: cytohesin 2
• NCBI Official Symbol: CYTH2
• NCBI Official Synonym Symbols: ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like
• NCBI Protein Information: cytohesin-2
• UniProt Protein Name: Cytohesin-2
• Protein Family: Cytohesin
• UniProt Gene Name: CYTH2
• UniProt Synonym Gene Names: ARNO; PSCD2; PSCD2L; Protein ARNO
• UniProt Entry Name: CYH2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

cytohesin 2: Acts as a guanine-nucleotide exchange factor (GEF). Promotes guanine-nucleotide exchange on ARF1, ARF3 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. The cell membrane form, in association with ARL4 proteins, recruits ARF6 to the plasma membrane. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, ARF; GEFs

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: membrane; cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: protein binding; lipid binding; ARF guanyl-nucleotide exchange factor activity

Biological Process: regulation of cell adhesion; vesicle-mediated transport; endocytosis; regulation of ARF protein signal transduction; actin cytoskeleton organization and biogenesis; positive regulation of GTPase activity