Rabbit DAP Kinase 1 Polyclonal Antibody | anti-DAPK1 antibody

Anti-DAP Kinase 1 Rabbit pAb

Cat. Num. AAA477989

• Gene Names: DAPK1; DAPK; ROCO3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Chromatography
• Synonyms: DAP Kinase 1; Polyclonal Antibody; Anti-DAP Kinase 1 Rabbit pAb; DAK1; DAP K1; DAP kinase 1; DAPK 1; DAPK; DAPK1_HUMAN; Death Associated Protein Kinase 1; Death-associated protein kinase 1; anti-DAPK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DAP Kinase 1 Antibody detects endogenous levels of total DAP Kinase 1
• Purity/Purification: Affinity Chromatography
• Form/Format: Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1430

• Applicable Applications for anti-DAPK1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC/IF: 1:50-1:200
• Immunogen: A synthesized peptide derived from human DAP Kinase 1
• Subcellular Location: Cell junction; Focal adhesion
• Preparation and Storage: Store at 4 degree C for short term.; Store at-20 degree C for long term.; Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot analysis of DAP Kinase 1 expression in HeLa cell lysate.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast cancer, using DAP Kinase 1 Antibody.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells, using DAP Kinase 1 Antibody.)

Related Product Information for anti-DAPK1 antibody

DAP (death associated protein) kinase and ZIP kinase are members of a novel protein kinase family, the members of which have the capacity to mediate apoptosis through their catalytic activities. DAP kinase (DAPK) contains a "death domain" and has been shown to mediate IFN-gamma-induced apoptosis. The introduction of DAPK into highly metastatic carcinoma clones lacking DAPK expression has been shown to result in the suppression of metastasis, thus linking suppression of apoptosis to metastasis.

NCBI and Uniprot Product Information

• NCBI GI #: 570359575
• NCBI GeneID: 1612
• NCBI Accession #: NP_001275658.1
• NCBI GenBank Nucleotide #: NM_001288729.1
• UniProt Accession #: P53355; Q14CQ7; Q1W5W0; Q68CP8; Q6ZRZ3; Q9BTL8; B7ZLD2; B7ZLE7
• Molecular Weight: 160kDa
• NCBI Official Full Name: death-associated protein kinase 1
• NCBI Official Synonym Full Names: death associated protein kinase 1
• NCBI Official Symbol: DAPK1
• NCBI Official Synonym Symbols: DAPK; ROCO3
• NCBI Protein Information: death-associated protein kinase 1
• UniProt Protein Name: Death-associated protein kinase 1
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK1
• UniProt Synonym Gene Names: DAPK; DAP kinase 1

NCBI Description

Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]

Uniprot Description

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.