KLK4 sirna

KLK4 siRNA (Human)

Cat. Num. AAA8229575

• Gene Names: KLK4; ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KLK4; KLK4 siRNA (Human); EMSP1; PRSS17; PSTS; Kallikrein-4; Enamel matrix serine proteinase 1; Kallikrein-like protein 1; KLK-L1; Prostase; Serine protease 17; KLK4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KLK4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 159

• Applicable Applications for KLK4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KLK4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KLK4 sirna

siRNA to inhibit KLK4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 729042255
• NCBI GeneID: 9622
• NCBI Accession #: NP_001289890.1
• NCBI GenBank Nucleotide #: NM_001302961.1
• UniProt Accession #: Q9Y5K2; Q4VB16; Q96RU5; Q9GZL6; Q9UBJ6
• Molecular Weight: 11,858 Da
• NCBI Official Full Name: kallikrein-4 isoform 2
• NCBI Official Synonym Full Names: kallikrein-related peptidase 4
• NCBI Official Symbol: KLK4
• NCBI Official Synonym Symbols: ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; kallikrein
• NCBI Protein Information: kallikrein-4
• UniProt Protein Name: Kallikrein-4
• Protein Family: Kallikrein
• UniProt Gene Name: KLK4
• UniProt Synonym Gene Names: EMSP1; PRSS17; PSTS; KLK-L1
• UniProt Entry Name: KLK4_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. In some tissues its expression is hormonally regulated. The expression pattern of a similar mouse protein in murine developing teeth supports a role for the protein in the degradation of enamel proteins. Several transcript variants encoding different proteins have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

KLK4: Involved in enamel formation. Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1). AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Secreted; Protease; EC 3.4.21.-; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region

Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; metal ion binding

Biological Process: extracellular matrix disassembly; protein catabolic process; proteolysis

Disease: Amelogenesis Imperfecta, Hypomaturation Type, Iia1