TMPRSS15 sirna

TMPRSS15 siRNA (Mouse)

Cat. Num. AAA8229192

• Gene Names: Tmprss15; Entk; Prss7
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TMPRSS15; TMPRSS15 siRNA (Mouse); ENTK; PRSS7; Enteropeptidase; Enterokinase; Serine protease 7; Transmembrane protease serine 15; TMPRSS15 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TMPRSS15 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1069

• Applicable Applications for TMPRSS15 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TMPRSS15 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TMPRSS15 sirna

siRNA to inhibit TMPRSS15 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6679489
• NCBI GeneID: 19146
• NCBI Accession #: NP_032967.1
• NCBI GenBank Nucleotide #: NM_008941.3
• UniProt Accession #: P97435; Q148Y3
• Molecular Weight: 118,735 Da
• NCBI Official Full Name: enteropeptidase isoform 1
• NCBI Official Synonym Full Names: transmembrane protease, serine 15
• NCBI Official Symbol: Tmprss15
• NCBI Official Synonym Symbols: Entk; Prss7
• NCBI Protein Information: enteropeptidase
• UniProt Protein Name: Enteropeptidase
• Protein Family: Enteropeptidase
• UniProt Gene Name: Tmprss15
• UniProt Synonym Gene Names: Entk; Prss7
• UniProt Entry Name: ENTK_MOUSE

NCBI Description

This gene encodes an enzyme that proteolytically activates the pancreatic proenzyme trypsinogen, converting it into trypsin. The encoded protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: Membrane protein, integral; EC 3.4.21.9; Protease

Cellular Component: membrane; integral to membrane

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity; scavenger receptor activity

Biological Process: proteolysis