KMT2C sirna

KMT2C siRNA (Human)

Cat. Num. AAA8228781

• Gene Names: KMT2C; HALR; MLL3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KMT2C; KMT2C siRNA (Human); HALR; KIAA1506; MLL3; Histone-lysine N-methyltransferase 2C; Lysine N-methyltransferase 2C; Homologous to ALR protein; Myeloid/lymphoid or mixed-lineage leukemia protein 3; KMT2C sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: KMT2C siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 4911

• Applicable Applications for KMT2C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human KMT2C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KMT2C sirna

siRNA to inhibit KMT2C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 91718902
• NCBI GeneID: 58508
• NCBI Accession #: NP_733751.2
• NCBI GenBank Nucleotide #: NM_170606.2
• UniProt Accession #: Q8NEZ4; Q8NC02; Q8NDF6; Q9H9P4; Q9NR13; Q9P222; Q9UDR7
• Molecular Weight: 547,633 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase 2C
• NCBI Official Synonym Full Names: lysine (K)-specific methyltransferase 2C
• NCBI Official Symbol: KMT2C
• NCBI Official Synonym Symbols: HALR; MLL3
• NCBI Protein Information: histone-lysine N-methyltransferase 2C
• UniProt Protein Name: Histone-lysine N-methyltransferase 2C
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: KMT2C
• UniProt Synonym Gene Names: HALR; KIAA1506; MLL3; Lysine N-methyltransferase 2C
• UniProt Entry Name: KMT2C_HUMAN

NCBI Description

This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a nuclear protein with an AT hook DNA-binding domain, a DHHC-type zinc finger, six PHD-type zinc fingers, a SET domain, a post-SET domain and a RING-type zinc finger. This protein is a member of the ASC-2/NCOA6 complex (ASCOM), which possesses histone methylation activity and is involved in transcriptional coactivation. [provided by RefSeq, Jul 2008]

Uniprot Description

MLL3: a protein methyltransferase that methylates K4 of histone H3. H3K4 methylation represents a specific tag for epigenetic transcriptional activation. Found in a critical region of chromosome 7, which is commonly deleted in malignant myeloid disorders. Partial duplication of the MLL3 gene are found in the juxtacentromeric region of chromosomes 1, 2, 13 and 21. Central component of the MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional coactivation. MLL3 may be a catalytic subunit of this complex. Contributes to genome-scale circadian transcription. May be involved in leukemogenesis and developmental disorder. Highly expressed in testis and ovary, followed by brain and liver. Also expressed in placenta, peripherical blood, fetal thymus, heart, lung and kidney. Within brain, expression was highest in hippocampus, caudate nucleus, and substantia nigra. Not detected in skeletal muscle and fetal liver. Belongs to the protein lysine methyltransferase family. TRX/MLL subfamily. Three isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K4 specific)

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; histone H3-K4 methylation