HTR3A sirna

HTR3A siRNA (Rat)

Cat. Num. AAA8226880

• Gene Names: Htr3a; 5HT3; Htr3; 5-HT3; 5-HT3A
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HTR3A; HTR3A siRNA (Rat); 5HT3; HTR3; 5-hydroxytryptamine receptor 3A; 5-HT3-A; 5-HT3A; 5-hydroxytryptamine receptor 3; 5-HT-3; 5-HT3R; Serotonin receptor 3A; Serotonin-gated ion channel receptor; HTR3A sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: HTR3A siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 483

• Applicable Applications for HTR3A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat HTR3A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HTR3A sirna

siRNA to inhibit HTR3A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 139947611
• NCBI GeneID: 79246
• NCBI Accession #: NP_077370.2
• NCBI GenBank Nucleotide #: NM_024394.2
• UniProt Accession #: P35563
• Molecular Weight: 55,428 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 3A
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic
• NCBI Official Symbol: Htr3a
• NCBI Official Synonym Symbols: 5HT3; Htr3; 5-HT3; 5-HT3A
• NCBI Protein Information: 5-hydroxytryptamine receptor 3A
• UniProt Protein Name: 5-hydroxytryptamine receptor 3A
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: Htr3a
• UniProt Synonym Gene Names: 5ht3; Htr3; 5-HT3-A; 5-HT3A; 5-HT-3; 5-HT3R
• UniProt Entry Name: 5HT3A_RAT

NCBI Description

receptor for 5-hydroxytryptamine (serotonin); functions as a ligand-gated ion channel [RGD, Feb 2006]

Uniprot Description

5-HT(3): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, cation; Channel, ligand-gated; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: postsynaptic membrane; cell soma; integral to plasma membrane; axon; cytoplasm; plasma membrane; cell junction

Molecular Function: voltage-gated potassium channel activity; serotonin-activated cation-selective channel activity; serotonin binding; serotonin receptor activity

Biological Process: synaptic transmission; response to ethanol; response to cocaine; cation transport; serotonin receptor signaling pathway