Typical Testing Data/Standard Curve (for reference only)
Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit | ADH1 elisa kit
Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit
Gene Names
ADH1; ADH; ADH1; ALCOHOL DEHYDROGENASE; alcohol dehydrogenase 1; ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE; ATADH; ATADH1; F22K20.19; F22K20_19
Reactivity
Human
Synonyms
Alcohol Dehydrogenase 1 (ADH1); Human Alcohol Dehydrogenase 1 (ADH1) ELISA Kit; ADH1A; Alcohol Dehydrogenase 1A(Class I)Alpha Polypeptide; Alcohol dehydrogenase subunit alpha; ADH1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Alcohol Dehydrogenase 1 (ADH1).
No significant cross-reactivity or interference between Alcohol Dehydrogenase 1 (ADH1) and analogues was observed.
No significant cross-reactivity or interference between Alcohol Dehydrogenase 1 (ADH1) and analogues was observed.
Sequence Length
379
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range
1.56-100ng/mL
Sensitivity
< 0.72ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Alcohol Dehydrogenase 1 (ADH1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ADH1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alcohol Dehydrogenase 1 (ADH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alcohol Dehydrogenase 1 (ADH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alcohol Dehydrogenase 1 (ADH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alcohol Dehydrogenase 1 (ADH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for ADH1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,178 Da
NCBI Official Full Name
alcohol dehydrogenase 1
NCBI Official Symbol
ADH1
NCBI Official Synonym Symbols
ADH; ADH1; ALCOHOL DEHYDROGENASE; alcohol dehydrogenase 1; ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE; ATADH; ATADH1; F22K20.19; F22K20_19
NCBI Protein Information
alcohol dehydrogenase 1
UniProt Protein Name
Alcohol dehydrogenase class-P
Protein Family
UniProt Gene Name
ADH1
UniProt Synonym Gene Names
ADH
UniProt Entry Name
ADH1_ARATH
NCBI Description
Catalyzes the reduction of acetaldehyde using NADH as reductant. Requires zinc for activity. Dimer. Anaerobic response polypeptide (ANP). Fermentation. The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
Uniprot Description
Alcohol dehydrogenase mostly active on ethanol (EtOH), but exhibits broad substrates selectivity for primary and secondary alcohols (e.g. butanol, propyl alcohol, pentanol, isopentanol, ethylene glycol, isopropanol, methanol and tertiary butyl alcohol) (PubMed:23707506). Converts allyl alcohol to highly toxic acryl-aldehyde (PubMed:20508152). Required for survival and acclimation in hypoxic conditions, especially in roots (PubMed:12857811, PubMed:9880346).
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Product Notes
The Human ADH1 adh1 (Catalog #AAA2020332) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2020332 ELISA Kit recognizes Human ADH1. It is sometimes possible for the material contained within the vial of "Alcohol Dehydrogenase 1 (ADH1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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