PLD6 sirna

PLD6 siRNA (Human)

Cat. Num. AAA8226546

• Gene Names: PLD6; ZUC
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLD6; PLD6 siRNA (Human); Mitochondrial cardiolipin hydrolase; Choline phosphatase 6; Mitochondrial phospholipase; MitoPLD; Phosphatidylcholine-hydrolyzing phospholipase D6; Phospholipase D6; PLD 6; Protein zucchini homolog; PLD6 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PLD6 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 252

• Applicable Applications for PLD6 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PLD6 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLD6 sirna

siRNA to inhibit PLD6 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 270265812
• NCBI GeneID: 201164
• NCBI Accession #: NP_849158.2
• NCBI GenBank Nucleotide #: NM_178836.3
• UniProt Accession #: Q8N2A8; Q8N5Y1
• Molecular Weight: 28,273 Da
• NCBI Official Full Name: mitochondrial cardiolipin hydrolase
• NCBI Official Synonym Full Names: phospholipase D family, member 6
• NCBI Official Symbol: PLD6
• NCBI Official Synonym Symbols: ZUC
• NCBI Protein Information: mitochondrial cardiolipin hydrolase
• UniProt Protein Name: Mitochondrial cardiolipin hydrolase
• Protein Family: Mitochondrial cardiolipin hydrolase
• UniProt Gene Name: PLD6
• UniProt Synonym Gene Names: MitoPLD; PLD 6
• UniProt Entry Name: PLD6_HUMAN

Uniprot Description

PLD6: Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily.

Protein type: Membrane protein, integral; EC 3.1.4.4; EC 3.1.4.-; Phospholipase

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrial outer membrane; integral to membrane

Molecular Function: protein homodimerization activity; endonuclease activity; metal ion binding

Biological Process: meiosis; polar granule organization and biogenesis; mitochondrial fusion; phosphatidylglycerol biosynthetic process; DNA methylation during gametogenesis; phospholipid metabolic process; glycerophospholipid biosynthetic process; gene expression; spermatid development; lipid catabolic process