CTNNB1 sirna

CTNNB1 siRNA (Rat)

Cat. Num. AAA8226393

• Gene Names: Ctnnb1; Catnb
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTNNB1; CTNNB1 siRNA (Rat); CATNB; Catenin beta-1; Beta-catenin; CTNNB1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: CTNNB1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 781

• Applicable Applications for CTNNB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat CTNNB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTNNB1 sirna

siRNA to inhibit CTNNB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 46048609
• NCBI GeneID: 84353
• NCBI Accession #: NP_445809.2
• NCBI GenBank Nucleotide #: NM_053357.2
• UniProt Accession #: Q9WU82
• Molecular Weight: 85,455 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin associated protein), beta 1
• NCBI Official Symbol: Ctnnb1
• NCBI Official Synonym Symbols: Catnb
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: Ctnnb1
• UniProt Synonym Gene Names: Catnb
• UniProt Entry Name: CTNB1_RAT

NCBI Description

involved in mediating the interaction between cadherins and the actin cytoskeleton [RGD, Feb 2006]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Oncoprotein; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; Actin-binding; Cell adhesion

Cellular Component: centrosome; basolateral plasma membrane; intercellular junction; fascia adherens; beta-catenin destruction complex; cytosol; transcription factor complex; cell-cell adherens junction; membrane; cell projection membrane; lamellipodium; perinuclear region of cytoplasm; cytoplasm; synapse; dendritic shaft; lateral plasma membrane; spindle pole; apical junction complex; neuron projection; focal adhesion; tight junction; protein complex; cell; catenin complex; cell cortex; Z disc; adherens junction; apical part of cell; microvillus membrane; plasma membrane; cell junction; nucleus

Molecular Function: protein C-terminus binding; transcription coactivator activity; protein phosphatase binding; transcription factor binding; ionotropic glutamate receptor binding; signal transducer activity; protein binding; enzyme binding; DNA binding; cadherin binding; double-stranded DNA binding; protein complex binding; estrogen receptor binding; chromatin binding; nitric-oxide synthase binding; SMAD binding; kinase binding; transcription factor activity; alpha-catenin binding; nuclear hormone receptor binding

Biological Process: skin development; cellular morphogenesis during differentiation; regulation of myelination; regulation of centriole-centriole cohesion; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; cell maturation; negative regulation of chondrocyte differentiation; positive regulation of fibroblast growth factor receptor signaling pathway; cell fate determination; morphogenesis of embryonic epithelium; chromatin-mediated maintenance of transcription; regulation of cell fate specification; positive regulation of mesenchymal cell proliferation; embryonic foregut morphogenesis; synapse organization and biogenesis; male genitalia development; kidney development; T cell differentiation; response to drug; positive regulation of neuroblast proliferation; transcription, DNA-dependent; regulation of smooth muscle cell proliferation; regulation of osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; regulation of gene expression; response to estrogen stimulus; regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; embryonic digit morphogenesis; embryonic forelimb morphogenesis; limb development; response to hormone stimulus; neuron migration; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; forebrain development; pancreas development; vasculogenesis; proximal/distal pattern formation; negative regulation of mitotic cell cycle, embryonic; Wnt receptor signaling pathway; in utero embryonic development; neural plate development; gastrulation with mouth forming second; positive regulation of telomere maintenance via telomerase; odontogenesis of dentine-containing teeth; regulation of cell proliferation; cell proliferation; positive regulation of osteoblast differentiation; Schwann cell proliferation; negative regulation of cell differentiation; response to cadmium ion; dorsal/ventral pattern formation; ureteric bud branching; embryonic heart tube development; lens morphogenesis in camera-type eye; lung development; anterior/posterior axis specification; protein heterooligomerization; heart development; T cell differentiation in the thymus; osteoclast differentiation; Wnt receptor signaling pathway through beta-catenin; response to organic cyclic substance; regulation of apoptosis; hair cycle process; cell-cell adhesion; positive regulation of endothelial cell differentiation; organ development; ectoderm development; positive regulation of epithelial cell differentiation; cell adhesion; bone resorption; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of protein sumoylation; patterning of blood vessels; genitalia morphogenesis; endoderm formation; midgut development; negative regulation of transcription, DNA-dependent; cellular process; oocyte development; negative regulation of osteoclast differentiation; glial cell fate determination; endodermal cell fate commitment; cell-matrix adhesion; cell fate specification; embryonic hindlimb morphogenesis; response to estradiol stimulus; synaptic transmission; central nervous system vasculogenesis; regulation of osteoclast differentiation; regulation of transcription, DNA-dependent; vasculature development; regulation of cell differentiation; hemopoiesis; cell differentiation; fallopian tube development; skeletal development; layer formation in the cerebral cortex; hair follicle morphogenesis; thymus development; embryonic axis specification; regulation of T cell proliferation; synaptic vesicle transport; positive regulation of telomerase activity; liver development; myoblast differentiation; negative regulation of oligodendrocyte differentiation; response to cytokine stimulus