Typical Testing Data/Standard Curve (for reference only)
Human Semaphorin 3D (SEMA3D) ELISA Kit | SEMA3D elisa kit
Human Semaphorin 3D (SEMA3D) ELISA Kit
Gene Names
SEMA3D; coll-2; Sema-Z2
Reactivity
Human
Synonyms
Semaphorin 3D (SEMA3D); Human Semaphorin 3D (SEMA3D) ELISA Kit; SEMA3D elisa kit
Reactivity
Human
Sequence Length
777
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.2ng/ml - 60ng/ml
Sensitivity
0.12ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA3D elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Semaphorin 3D (also known as Sema3d) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Sema3d antibody. Sema3d present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Sema3d Antibody is added and binds to Sema3d in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Sema3d antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Sema3d. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human Sema3d antibody. Sema3d present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human Sema3d Antibody is added and binds to Sema3d in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Sema3d antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human Sema3d. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,651 Da
NCBI Official Full Name
semaphorin-3D
NCBI Official Synonym Full Names
semaphorin 3D
NCBI Official Symbol
SEMA3D
NCBI Official Synonym Symbols
coll-2; Sema-Z2
NCBI Protein Information
semaphorin-3D
UniProt Protein Name
Semaphorin-3D
Protein Family
UniProt Gene Name
SEMA3D
NCBI Description
This gene encodes a member of the semaphorin III family of secreted signaling proteins that are involved in axon guidance during neuronal development. The encoded protein contains an N-terminal Sema domain, an immunoglobulin like domain and a C-terminal basic domain. The protein encoded by this gene binds neuropilin and plays an important role in cardiovascular development. [provided by RefSeq, Aug 2016]
Uniprot Description
Induces the collapse and paralysis of neuronal growth cones. Could potentially act as repulsive cues toward specific neuronal populations. Binds to neuropilin ().
Research Articles on SEMA3D
Similar Products
Product Notes
The Human SEMA3D sema3d (Catalog #AAA164087) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA164087 ELISA Kit recognizes Human SEMA3D. It is sometimes possible for the material contained within the vial of "Semaphorin 3D (SEMA3D), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
