MAD2 Blocking Peptide | MAD2L1 blocking peptide

MAD2 Blocking Peptide

Cat. Num. AAA822605

• Gene Names: MAD2L1; MAD2; HSMAD2
• Reactivity: Human, Mouse, Rat, Monkey
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: MAD2; MAD2 Blocking Peptide; Mitotic spindle assembly checkpoint protein MAD2A; HsMAD2; Mitotic arrest deficient 2-like protein 1; MAD2-like protein 1; MAD2L1 blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Rat, Monkey
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 205

• Applicable Applications for MAD2L1 blocking peptide: Blocking (BL)
• Application Notes: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAD2L1 blocking peptide

The peptide is used to block Anti-MAD2 Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 4505067
• NCBI GeneID: 4085
• NCBI Accession #: NP_002349.1
• NCBI GenBank Nucleotide #: NM_002358.3
• UniProt Accession #: Q13257; Q53F56; Q548X9; Q6IRW7; Q8IZX3
• Molecular Weight: 10,335 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD2A
• NCBI Official Synonym Full Names: MAD2 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD2L1
• NCBI Official Synonym Symbols: MAD2; HSMAD2
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD2A; MAD2-like protein 1; mitotic arrest deficient 2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD2A
• Protein Family: MAD2L1-binding protein
• UniProt Gene Name: MAD2L1
• UniProt Synonym Gene Names: MAD2; HsMAD2; MAD2-like protein 1
• UniProt Entry Name: MD2L1_HUMAN

NCBI Description

MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD2L1: required for the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. Inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. Interacts with Cdc20, MAD2L1BP and with ADAM17/TACE

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: kinetochore; spindle pole; perinuclear region of cytoplasm; nuclear pore; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; protein homodimerization activity

Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; negative regulation of mitotic cell cycle; mitotic cell cycle; negative regulation of protein catabolic process; negative regulation of apoptosis