DLG4 sirna

DLG4 siRNA (Mouse)

Cat. Num. AAA8225822

• Gene Names: Dlg4; Dlgh4; PSD95; SAP90; PSD-95; SAP90A
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DLG4; DLG4 siRNA (Mouse); DLGH4; PSD95; Disks large homolog 4; Postsynaptic density protein 95; PSD-95; Synapse-associated protein 90; SAP-90; SAP90; DLG4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DLG4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 721

• Applicable Applications for DLG4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DLG4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DLG4 sirna

siRNA to inhibit DLG4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 157909820
• NCBI GeneID: 13385
• NCBI Accession #: NP_001103222.1
• NCBI GenBank Nucleotide #: NM_001109752.1
• UniProt Accession #: Q62108; Q5NCV5; Q5NCV6; Q5NCV7; Q91WJ1
• Molecular Weight: 80,102 Da
• NCBI Official Full Name: disks large homolog 4 isoform 2
• NCBI Official Synonym Full Names: discs, large homolog 4 (Drosophila)
• NCBI Official Symbol: Dlg4
• NCBI Official Synonym Symbols: Dlgh4; PSD95; SAP90; PSD-95; SAP90A
• NCBI Protein Information: disks large homolog 4
• UniProt Protein Name: Disks large homolog 4
• Protein Family: Disks large
• UniProt Gene Name: Dlg4
• UniProt Synonym Gene Names: Dlgh4; Psd95; PSD-95; SAP-90; SAP90
• UniProt Entry Name: DLG4_MOUSE

Uniprot Description

PSD-95: a membrane associated guanylate kinase (MAGUK) scaffolding protein located in neural postsynaptic densities. Associates with NMDA receptor NR2 subunits via glutamate serine (aspartate/glutamate) valine motifs in the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Its overexpression or depletion changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. Binds tissue-type plasminogen activator (tPA), mediating neural NMDA gating via a complex of LRP1, PSD-95 and NMDAR. Interacts through its first two PDZ domains with NMDAR2A, NMDAR2B, NMDAR2C, NMDAR2D, ACCN3, certain splice forms of NMDAR1, KV4.2, CXADR and synGAP. Interacts through its first two PDZ domains with Kv1.1, Kv1.2, Kv1.3, Kv1.4 and HER4. Interacts through its first PDZ domain with GluR6 and Kv1.4. Interacts through its second PDZ domain with the PDZ domain of nNOS or the C-terminus of NOS1AP. May interact with 5-HT(2A). Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with SAPAP1, SAPAP2, SAPAP3, SAPAP4, MAP1A, and BEGAIN. Interacts through its guanylate kinase-like domain with KIF13B. Two alternatively spliced human isoforms have been reported. Palmitoylation of isoform 1 is required for targeting to postsynaptic density.

Protein type: Adaptor/scaffold

Cellular Component: cortical cytoskeleton; dendrite cytoplasm; voltage-gated potassium channel complex; endoplasmic reticulum; dendrite; postsynaptic density; dendritic spine; excitatory synapse; cytosol; postsynaptic membrane; synaptic vesicle; extrinsic to internal side of plasma membrane; cell projection; membrane; cytoplasm; plasma membrane; ionotropic glutamate receptor complex; synapse; cell junction

Molecular Function: protein C-terminus binding; P2Y1 nucleotide receptor binding; glutamate receptor binding; beta-1 adrenergic receptor binding; acetylcholine receptor binding; protein kinase binding; protein phosphatase binding; PDZ domain binding; ionotropic glutamate receptor binding; guanylate kinase activity; protein binding; neurexin binding; protein complex binding; structural molecule activity; D1 dopamine receptor binding; kinase binding; receptor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; locomotory behavior; social behavior; signal transduction; response to cocaine; positive regulation of synaptic transmission; establishment of protein localization; elevation of cytosolic calcium ion concentration; protein complex assembly; neuromuscular process controlling balance; negative regulation of receptor internalization; regulation of neuronal synaptic plasticity; nucleotide phosphorylation