APEX1 sirna

APEX1 siRNA (Mouse)

Cat. Num. AAA8225561

• Gene Names: Apex1; APE; Apex; HAP1; Ref-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: APEX1; APEX1 siRNA (Mouse); APE; APEX; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; REF-1; Redox factor-1; APEX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: APEX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 317

• Applicable Applications for APEX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse APEX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for APEX1 sirna

siRNA to inhibit APEX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6753086
• NCBI GeneID: 11792
• NCBI Accession #: NP_033817.1
• NCBI GenBank Nucleotide #: NM_009687.2
• UniProt Accession #: P28352
• Molecular Weight: 35,490 Da
• NCBI Official Full Name: DNA-(apurinic or apyrimidinic site) lyase
• NCBI Official Synonym Full Names: apurinic/apyrimidinic endonuclease 1
• NCBI Official Symbol: Apex1
• NCBI Official Synonym Symbols: APE; Apex; HAP1; Ref-1
• NCBI Protein Information: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Protein Name: DNA-(apurinic or apyrimidinic site) lyase
• Protein Family: DNA-(apurinic or apyrimidinic site) lyase
• UniProt Gene Name: Apex1
• UniProt Synonym Gene Names: Ape; Apex; Ref1; APEN; AP endonuclease 1
• UniProt Entry Name: APEX1_MOUSE

Uniprot Description

APE1: a multifunctional enzyme that plays a central role in the cellular response to oxidative stress including DNA repair and redox regulation of transcriptional factors. Binds DNA and RNA. Functions as an apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway, a 3'-5' exoribonuclease for mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules, and a DNA 3' phosphodiesterase capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. Is a loading factor for POLB onto non-incised AP sites in DNA, stimulates the 5'-terminal deoxyribose 5'- phosphate (dRp) excision activity of POLB, and involved in the DNA cleavage step of class switch recombination (CSR). Possesses reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Binds to negative calcium response elements (nCaREs). Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Is an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover. In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Interacts with SIRT1; the interaction is increased in the context of genotoxic stress. Interacts with HDAC1, HDAC2 and HDAC3; the interactions are not dependent on the APEX1 acetylation status. Up-regulated in presence of reactive oxygen species (ROS), like bleomycin, H2O2 and phenazine methosulfate. NPM1 stimulates endodeoxyribonuclease activity on double-stranded DNA with AP sites, but inhibits endoribonuclease activity on single-stranded RNA containing AP sites. Belongs to the DNA repair enzymes AP/ExoA family.

Protein type: DNA-binding; EC 4.2.99.18; Nuclear receptor co-regulator; Nucleolus; Endoplasmic reticulum; Transcription, coactivator/corepressor; Lyase; Deoxyribonuclease; DNA repair, damage; Hydrolase

Cellular Component: nucleoplasm; centrosome; transcription factor complex; mitochondrion; perinuclear region of cytoplasm; endoplasmic reticulum; cytoplasm; nucleolus; nuclear speck; intracellular; nucleus

Molecular Function: DNA-(apurinic or apyrimidinic site) lyase activity; nuclease activity; hydrolase activity; RNA binding; chromatin DNA binding; metal ion binding; transcription coactivator activity; lyase activity; oxidoreductase activity; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity; endoribonuclease activity; NF-kappaB binding; DNA binding; endonuclease activity; exonuclease activity; damaged DNA binding; protein complex binding; 3'-5' exonuclease activity; double-stranded DNA specific 3'-5' exodeoxyribonuclease activity; site-specific endodeoxyribonuclease activity, specific for altered base

Biological Process: positive regulation of DNA repair; transcription, DNA-dependent; negative regulation of smooth muscle cell migration; DNA repair; regulation of mRNA stability; DNA catabolic process, endonucleolytic; DNA recombination; regulation of transcription, DNA-dependent; base-excision repair; cell redox homeostasis; DNA catabolic process, exonucleolytic; response to DNA damage stimulus; aging