ZNRF1 sirna

ZNRF1 siRNA (Human)

Cat. Num. AAA8224801

• Gene Names: ZNRF1; NIN283
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZNRF1; ZNRF1 siRNA (Human); NIN283; E3 ubiquitin-protein ligase ZNRF1; Nerve injury-induced gene 283 protein; Zinc/RING finger protein 1; ZNRF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZNRF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 227

• Applicable Applications for ZNRF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZNRF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZNRF1 sirna

siRNA to inhibit ZNRF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14150005
• NCBI GeneID: 84937
• NCBI Accession #: NP_115644.1
• NCBI GenBank Nucleotide #: NM_032268.4
• UniProt Accession #: Q8ND25; Q9H083; D3DUJ9
• Molecular Weight: 29,777 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase ZNRF1
• NCBI Official Synonym Full Names: zinc and ring finger 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: ZNRF1
• NCBI Official Synonym Symbols: NIN283
• NCBI Protein Information: E3 ubiquitin-protein ligase ZNRF1
• UniProt Protein Name: E3 ubiquitin-protein ligase ZNRF1
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: ZNRF1
• UniProt Synonym Gene Names: NIN283
• UniProt Entry Name: ZNRF1_HUMAN

NCBI Description

This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]

Uniprot Description

ZNRF1: E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: synaptic vesicle membrane; membrane; lysosome; cell junction; cytosol; endosome

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process