ROR1 sirna

ROR1 siRNA (Human)

Cat. Num. AAA8224465

• Gene Names: ROR1; NTRKR1; dJ537F10.1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ROR1; ROR1 siRNA (Human); NTRKR1; Tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase. receptor-related 1; ROR1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ROR1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 393

• Applicable Applications for ROR1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ROR1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ROR1 sirna

siRNA to inhibit ROR1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 134152686
• NCBI GeneID: 4919
• NCBI Accession #: NP_001077061.1
• NCBI GenBank Nucleotide #: NM_001083592.1
• UniProt Accession #: Q01973; Q5VVX6; Q66K77; Q92776
• Molecular Weight: 43,826 Da
• NCBI Official Full Name: tyrosine-protein kinase transmembrane receptor ROR1 isoform 2
• NCBI Official Synonym Full Names: receptor tyrosine kinase-like orphan receptor 1
• NCBI Official Symbol: ROR1
• NCBI Official Synonym Symbols: NTRKR1; dJ537F10.1
• NCBI Protein Information: tyrosine-protein kinase transmembrane receptor ROR1
• UniProt Protein Name: Tyrosine-protein kinase transmembrane receptor ROR1
• Protein Family: Inactive tyrosine-protein kinase transmembrane receptor
• UniProt Gene Name: ROR1
• UniProt Synonym Gene Names: NTRKR1
• UniProt Entry Name: ROR1_HUMAN

NCBI Description

This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012]

Uniprot Description

ROR1: Tyrosine-protein kinase receptor whose role is not yet clear. Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Protein kinase, TK; Membrane protein, integral; Kinase, protein; TK group; Ror family

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: integral to plasma membrane; cytoplasm; plasma membrane; receptor complex

Molecular Function: Wnt-protein binding; protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway