FAF1 sirna

FAF1 siRNA (Human)

Cat. Num. AAA8224449

• Gene Names: FAF1; hFAF1; CGI-03; HFAF1s; UBXD12; UBXN3A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FAF1; FAF1 siRNA (Human); UBXD12; UBXN3A; FAS-associated factor 1; hFAF1; UBX domain-containing protein 12; UBX domain-containing protein 3A; FAF1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FAF1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 650

• Applicable Applications for FAF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FAF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FAF1 sirna

siRNA to inhibit FAF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 5901948
• NCBI GeneID: 11124
• NCBI Accession #: NP_008982.1
• NCBI GenBank Nucleotide #: NM_007051.2
• UniProt Accession #: Q9UNN5; Q549F0; Q9UF34; Q9UNT3; Q9Y2Z3
• Molecular Weight: 56,934 Da
• NCBI Official Full Name: FAS-associated factor 1
• NCBI Official Synonym Full Names: Fas (TNFRSF6) associated factor 1
• NCBI Official Symbol: FAF1
• NCBI Official Synonym Symbols: hFAF1; CGI-03; HFAF1s; UBXD12; UBXN3A
• NCBI Protein Information: FAS-associated factor 1
• UniProt Protein Name: FAS-associated factor 1
• Protein Family: FAS-associated factor
• UniProt Gene Name: FAF1
• UniProt Synonym Gene Names: UBXD12; UBXN3A; hFAF1
• UniProt Entry Name: FAF1_HUMAN

NCBI Description

Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain. [provided by RefSeq, Jul 2008]

Uniprot Description

FAF1: a pro-apoptotic protein that binds to FAS antigen. Potentiates but cannot initiate FAS-induced apoptosis. Its pro-apoptotic activity requires a ubiquitin-like domain but not the FAS-binding domain. Phosphorylation by protein kinase CK2 influences transport into nucleus. Two transcript variants encoding different protein isoforms have been described.

Protein type: Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p33

Cellular Component: perinuclear region of cytoplasm; CD95 death-inducing signaling complex; nuclear envelope; cytosol; nucleus

Molecular Function: protein domain specific binding; NF-kappaB binding; ubiquitin binding; protein binding; ubiquitin protein ligase binding; heat shock protein binding; protein kinase regulator activity; protein kinase binding

Biological Process: cell death; regulation of cell adhesion; proteasomal ubiquitin-dependent protein catabolic process; cytoplasmic sequestering of NF-kappaB; positive regulation of protein complex assembly; positive regulation of apoptosis; apoptosis; regulation of protein catabolic process; regulation of protein kinase activity