CCL4L2 sirna

CCL4L2 siRNA (Human)

Cat. Num. AAA8224419

• Gene Names: CCL4L2; CCL4L; SCYA4L; AT744.2; SCYQ4L2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCL4L2; CCL4L2 siRNA (Human); CCL4L; LAG1; SCYA4L1; SCYA4L2; C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; LAG-1; Macrophage inflammatory protein 1-beta; MIP-1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; CCL4L2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CCL4L2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 87

• Applicable Applications for CCL4L2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCL4L2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCL4L2 sirna

siRNA to inhibit CCL4L2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 612400620
• NCBI GeneID: 9560
• NCBI Accession #: NP_001278397.1
• NCBI GenBank Nucleotide #: NM_001291468.1
• UniProt Accession #: Q8NHW4; Q50EM1; Q50EM2; Q50EM3; Q50EM4; Q50EM5; Q50EM6; Q50EM7; Q50EM8; Q569J2; B2RUZ3; B7ZMA8
• Molecular Weight: 11,008 Da
• NCBI Official Full Name: C-C motif chemokine 4-like isoform 1
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 4-like 2
• NCBI Official Symbol: CCL4L2
• NCBI Official Synonym Symbols: CCL4L; SCYA4L; AT744.2; SCYQ4L2
• NCBI Protein Information: C-C motif chemokine 4-like
• UniProt Protein Name: C-C motif chemokine 4-like
• UniProt Gene Name: CCL4L1
• UniProt Synonym Gene Names: CCL4L; LAG1; SCYA4L1; LAG-1; MIP-1-beta
• UniProt Entry Name: CC4L_HUMAN

NCBI Description

This gene is one of several cytokine genes that are clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins that function in inflammatory and immunoregulatory processes. The protein encoded by this family member is similar to the chemokine (C-C motif) ligand 4 product, which inhibits HIV entry by binding to the cellular receptor CCR5. The copy number of this gene varies among individuals, where most individuals have one to five copies. This gene copy contains a non-consensus splice acceptor site at the 3' terminal exon found in other highly similar gene copies, and it thus uses other alternative splice sites for the 3' terminal exon, resulting in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CCL4L1: Chemokine that induces chemotaxis of cells expressing CCR5 or CCR1. Inhibits HIV replication in peripheral blood monocytes that express CCR5. Belongs to the intercrine beta (chemokine CC) family. 10 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Chemokine; Secreted

Chromosomal Location of Human Ortholog: 17q12