AGTR1B sirna

AGTR1B siRNA (Rat)

Cat. Num. AAA8224005

• Gene Names: Agtr1b; AT1; AT3; AT1B; Agtr1; AT₁R
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGTR1B; AGTR1B siRNA (Rat); AGTR1; AT1B; Type-1B angiotensin II receptor; AT3; Angiotensin II type-1B receptor; AGTR1B sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: AGTR1B siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 359

• Applicable Applications for AGTR1B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat AGTR1B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGTR1B sirna

siRNA to inhibit AGTR1B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 82524858
• NCBI GeneID: 81638
• NCBI Accession #: NP_112271.2
• NCBI GenBank Nucleotide #: NM_031009.2
• UniProt Accession #: P29089
• Molecular Weight: 40,912 Da
• NCBI Official Full Name: type-1B angiotensin II receptor
• NCBI Official Synonym Full Names: angiotensin II receptor, type 1b
• NCBI Official Symbol: Agtr1b
• NCBI Official Synonym Symbols: AT1; AT3; AT1B; Agtr1; AT₁R
• NCBI Protein Information: type-1B angiotensin II receptor
• UniProt Protein Name: Type-1B angiotensin II receptor
• Protein Family: Type-1B angiotensin II receptor
• UniProt Gene Name: Agtr1b
• UniProt Synonym Gene Names: Agtr1; At1b; AT1B
• UniProt Entry Name: AGTRB_RAT

NCBI Description

G-protein coupled receptor for angiotensin II; has a role in blood pressure regulation, electrolyte balance and cardiac function [RGD, Feb 2006]

Uniprot Description

AGTR1B: Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, integral

Cellular Component: membrane; basolateral plasma membrane; integral to membrane; plasma membrane; vesicle membrane; cytosol

Molecular Function: G-protein coupled receptor activity; signal transducer activity; protein self-association; angiotensin type II receptor activity; angiotensin type I receptor activity; peptide hormone binding

Biological Process: response to drug; blood vessel development; renal response to blood flow during renin-angiotensin regulation of systemic arterial blood pressure; drinking behavior; angiotensin mediated regulation of renal output; signal transduction; brain renin-angiotensin system; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; response to estrogen stimulus; regulation of vasoconstriction; response to hypoxia; negative regulation of neuron apoptosis; inflammatory response; kidney development; response to nutrient