CDK11B sirna

CDK11B siRNA (Human)

Cat. Num. AAA8223854

• Gene Names: CDK11B; p58; PK58; CDK11; CLK-1; CDC2L1; PITSLREA; p58CLK-1; CDK11-p46; CDK11-p58; p58CDC2L1; CDK11-p110
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CDK11B; CDK11B siRNA (Human); CDC2L1; CDK11; PITSLREA; PK58; Cyclin-dependent kinase 11B; Cell division cycle 2-like protein kinase 1; CLK-1; Cell division protein kinase 11B; Galactosyltransferase-associated protein kinase p58/GTA; PITSLRE serine/threonine-protein kinase CDC2L1; p58 CLK-1; CDK11B sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CDK11B siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 526

• Applicable Applications for CDK11B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CDK11B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CDK11B sirna

siRNA to inhibit CDK11B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 16332360
• NCBI GeneID: 984
• NCBI Accession #: NP_277022.1
• NCBI GenBank Nucleotide #: NM_033487.2
• UniProt Accession #: P21127; O95265; Q12817; Q12818; Q12819; Q12820; Q12822; Q8N530; Q9NZS5; Q9UBJ0; Q9UBQ1; Q9UBR0
• Molecular Weight: 49,555 Da
• NCBI Official Full Name: cyclin-dependent kinase 11B isoform 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase 11B
• NCBI Official Symbol: CDK11B
• NCBI Official Synonym Symbols: p58; PK58; CDK11; CLK-1; CDC2L1; PITSLREA; p58CLK-1; CDK11-p46; CDK11-p58; p58CDC2L1; CDK11-p110
• NCBI Protein Information: cyclin-dependent kinase 11B
• UniProt Protein Name: Cyclin-dependent kinase 11B
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK11B
• UniProt Synonym Gene Names: CDC2L1; CDK11; PITSLREA; PK58; CLK-1
• UniProt Entry Name: CD11B_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. Members of this kinase family are known to be essential for eukaryotic cell cycle control. Due to a segmental duplication, this gene shares very high sequence identity with a neighboring gene. These two genes are frequently deleted or altered in neuroblastoma. The protein kinase encoded by this gene can be cleaved by caspases and may play a role in cell apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]

Uniprot Description

CDK11B: a protein kinase of the CDK family. May act as a negative regulator of the normal cell cycle progression. Nine alternatively spliced isoforms have been described. The p110 isoforms have been suggested to be involved in pre-mRNA splicing, potentially by phosphorylating the splicing protein SFRS7. The p58 isoform is specifically induced in G2/M phase of the cell cycle. 2 isoforms, SV9 and 7, are produced by alternative initiation at Met-1 and Met-357 resulting in p110 and p58 isoforms, respectively. Deleted or altered frequently in neuroblastoma with amplified MYCN genes. Can be cleaved by caspases and demonstrated to play a role in cell apoptosis.

Protein type: Kinase, protein; Protein kinase, CMGC; Motility/polarity/chemotaxis; Cell cycle regulation; Apoptosis; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK/PITSLRE subfamily; PITSLRE subfamily

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; cyclin-dependent protein kinase activity; ATP binding; protein kinase activity

Biological Process: mitosis; cell proliferation; regulation of transcription, DNA-dependent; apoptosis; regulation of cell cycle; regulation of cell growth; protein amino acid phosphorylation; regulation of mRNA processing