LEO1 sirna

LEO1 siRNA (Mouse)

Cat. Num. AAA8222683

• Gene Names: Leo1; Gm185
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LEO1; LEO1 siRNA (Mouse); GM185; RNA polymerase-associated protein LEO1; LEO1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LEO1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 667

• Applicable Applications for LEO1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LEO1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LEO1 sirna

siRNA to inhibit LEO1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 87299619
• NCBI GeneID: 235497
• NCBI Accession #: NP_001034611.1
• NCBI GenBank Nucleotide #: NM_001039522.1
• UniProt Accession #: Q5XJE5; Q640R1; E9QPK8
• Molecular Weight: 35,869 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1
• NCBI Official Synonym Full Names: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: Leo1
• NCBI Official Synonym Symbols: Gm185
• NCBI Protein Information: RNA polymerase-associated protein LEO1
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: Leo1
• UniProt Synonym Gene Names: Gm185
• UniProt Entry Name: LEO1_MOUSE

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; negative regulation of myeloid cell differentiation; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; stem cell maintenance; histone H2B ubiquitination; positive regulation of mRNA 3'-end processing; positive regulation of transcription from RNA polymerase II promoter; mRNA polyadenylation