PRDM7 sirna

PRDM7 siRNA (Human)

Cat. Num. AAA8220077

• Gene Names: PRDM7; PFM4; ZNF910
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PRDM7; PRDM7 siRNA (Human); PFM4; Probable histone-lysine N-methyltransferase PRDM7; PR domain zinc finger protein 7; PR domain-containing protein 7; PRDM7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PRDM7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 492

• Applicable Applications for PRDM7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PRDM7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PRDM7 sirna

siRNA to inhibit PRDM7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148271100
• NCBI GeneID: 11105
• NCBI Accession #: NP_001091643.1
• NCBI GenBank Nucleotide #: NM_001098173.1
• UniProt Accession #: Q9NQW5; Q08EM4; Q9NQW4; A4Q9G8
• Molecular Weight: 45,890 Da
• NCBI Official Full Name: probable histone-lysine N-methyltransferase PRDM7
• NCBI Official Synonym Full Names: PR domain containing 7
• NCBI Official Symbol: PRDM7
• NCBI Official Synonym Symbols: PFM4; ZNF910
• NCBI Protein Information: probable histone-lysine N-methyltransferase PRDM7
• UniProt Protein Name: Probable histone-lysine N-methyltransferase PRDM7
• Protein Family: Probable histone-lysine N-methyltransferase
• UniProt Gene Name: PRDM7
• UniProt Synonym Gene Names: PFM4
• UniProt Entry Name: PRDM7_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that may have roles in transcription and other nuclear processes. The encoded protein contains a KRAB (Kruppel-associated box) domain -A box and a SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain and may function as a histone methyltransferase. [provided by RefSeq, Aug 2013]

Uniprot Description

PRDM7: a probable histone methyltransferase. The mouse orthologous protein seems not to exist. Human PRDM7 and PRDM9 genes, a pair of close paralogs corresponding to a single mouse gene Prdm9, may have been generated by a recent gene duplication event after the divergence of the ancestors of human and mouse. Three isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: chromosome; nucleus

Molecular Function: nucleic acid binding; histone-lysine N-methyltransferase activity

Biological Process: regulation of transcription, DNA-dependent