PLCE1 sirna

PLCE1 siRNA (Mouse)

Cat. Num. AAA8219373

• Gene Names: Plce1; Plce; mKIAA1516; PLCepsilon; 4933403A21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PLCE1; PLCE1 siRNA (Mouse); KIAA1516; PLCE; 1-phosphatidylinositol 4.5-bisphosphate phosphodiesterase epsilon-1; Phosphoinositide phospholipase C-epsilon-1; Phospholipase C-epsilon-1; PLC-epsilon-1; PLCE1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PLCE1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2282

• Applicable Applications for PLCE1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PLCE1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PLCE1 sirna

siRNA to inhibit PLCE1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 134053943
• NCBI GeneID: 74055
• NCBI Accession #: NP_062534.2
• NCBI GenBank Nucleotide #: NM_019588.2
• UniProt Accession #: Q8K4S1; Q3TS68; Q80TC4; Q8BZF3; Q9JKM2; B9EHS1; E9Q5G0
• Molecular Weight: 255,047 Da
• NCBI Official Full Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
• NCBI Official Synonym Full Names: phospholipase C, epsilon 1
• NCBI Official Symbol: Plce1
• NCBI Official Synonym Symbols: Plce; mKIAA1516; PLCepsilon; 4933403A21Rik
• NCBI Protein Information: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
• UniProt Protein Name: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1
• Protein Family: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase
• UniProt Gene Name: Plce1
• UniProt Synonym Gene Names: Kiaa1516; Plce; PLC-epsilon-1
• UniProt Entry Name: PLCE1_MOUSE

Uniprot Description

PLCE1: a widely expressed bifunctional enzyme with phospholipase activity and Ras guanine-exchange factor (RasGEF) activity. Catalyzes the release of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) from 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. Is an effector of heterotrimeric G protein activation. May play a role in cell survival, cell growth, actin organization and T-cell activation. Activated by the heterotrimeric G-protein subunits GNA12, GNA13 and GNB1-GNG2. Activated by HRAS, RAP1A, RHOA, RHOB, RHOC, RRAS and RRAS2. Activated by the G(s)-coupled GPCRs ADRB2, PTGER1 and CHRM3 through cyclic-AMP formation and RAP2B activation. Inhibited by G(i)-coupled GPCRs. Interacts with RHOA. Interacts with IQGAP1, HRAS, RAP1A, RAP2A, RAP2B and RRAS. Recruited to plasma membrane by activated HRAS and RAP2. Recruited to perinuclear membrane by activated RAP1A. Requires calcium for activiation. The Ras-associating domain 1 is degenerate and may not bind HRAS. The Ras-associating domain 2 mediates interaction with GTP-bound HRAS, RAP1A, RAP2A and RAP2B and recruitment of HRAS to the cell membrane. The Ras-GEF domain has a GEF activity towards HRAS and RAP1A. Mediates activation of the mitogen-activated protein kinase pathway. Defects in PLCE1 are the cause of nephrotic syndrome type 3 (NPHS3). Nephrotic syndrome, a malfunction of the kidney glomerular filter, leads to proteinuria, hypoalbuminemia, edema. Overexpressed during heart failure. A GWAS study identified an association between esophageal squamous cell carcinoma and a common DNA variant in the PLCE1 gene in East Asians. Two isoforms of the human protein are produced by alternative splicing. Isoform 1 is broadly expressed and only absent in peripheral blood leukocytes. Isoform 2 is specifically expressed in placenta, lung and spleen. Isoform 1 and isoform 2 associate with Golgi membranes.

Protein type: Carbohydrate Metabolism - inositol phosphate; GAPs; EC 3.1.4.11; GAPs, Ras; Phospholipase

Cellular Component: Golgi apparatus; membrane; cytoplasm; plasma membrane

Molecular Function: signal transducer activity; phosphoric diester hydrolase activity; hydrolase activity; guanyl-nucleotide exchange factor activity; phosphoinositide phospholipase C activity

Biological Process: small GTPase mediated signal transduction; lipid metabolic process; signal transduction; lipid catabolic process