COVID 19 Spike protein, Delta B.1.617.2 variant Coronavirus Recombinant Protein

Recombinant SARS-CoV-2 Spike protein, Delta B.1.617.2 variant (Indian variant, T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R and D950N)

Cat. Num. AAA184024

• Applications: Western Blot
• Purity: Affinity chromatography. >95%
• Synonyms: COVID 19 Spike protein; Delta B.1.617.2 variant Coronavirus; Recombinant SARS-CoV-2 Spike protein; Delta B.1.617.2 variant (Indian variant; T19R; G142D; EF156-157del; R158G; L452R; T478K; D614G; P681R and D950N); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; B.1.617.2 Variant of Concern; India mutant; Indian variant; P681R; D950N; Delta B.1.617.2 variant Coronavirus recombinant protein

• Host: HEK293 Cells
• Purity/Purification: Affinity chromatography. >95%
• Form/Format: Liquid
• Sequence Positions: 16-1211
• Sequence: VNLRTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLDYYHKNNKSWMESGVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGSKPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNSYECDIPIGAGICASYQTQTNSRRGAGSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSPIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGPALQIPFPMQMAYRFNGIGVTNVLYENQKLIANQFNSAIGKIQDSLSSTPSALGKLQNVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDSGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKGSGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGSGHHHHHHHH

• Applicable Applications for COVID 19 Spike protein, Delta B.1.617.2 variant Coronavirus recombinant protein: Western Blot (WB), Immunoassay (IA), Functional Studies
• Endotoxin: Less than 0.1 ng/ug (1 IEU/ug), as measured by LAL method.
• Tag Information: 8xHis at the C-terminus
• Buffer: PBS 20% Glycerol
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.

SDS-Page

(>90% as estimated by SDS-PAGE stained with Instant Blue Stain (Abcam).)

Related Product Information for COVID 19 Spike protein, Delta B.1.617.2 variant Coronavirus recombinant protein

Full-length soluble with foldon trimerization motif, mutated Furin recognition site and 6 stabilising mutations (F817P, A892P, A899P, A942P, K986P and V987P), based on/modified from Amanat et al, 2020 and Hsieh et al, 2020.

The first reports of atypical pneumonia in China were announced at the very end of 2019, in the city of Wuhan, Hubei province. Its cause was identified in January 2020 as a novel ?-CoV (1), named SARS-CoV-2, which started spreading very quickly causing a global pandemic COVID-19. Spike protein attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (2, 3). The protein forms a trimer in vivo mediating receptor binding and membrane fusion (4). It has been demonstrated by Florian Krammer’s group that certain mutations and the inclusion of trimerization motif can stabilize recombinant Spike protein (5, 6). The original Wuhan strain of the virus has become quickly replaced by its more transmissible variant, mainly determined by a single amino acid point mutation D614G (7). A novel SARS-CoV-2 variant, B.1.617, known as Indian variant, originated in India and quickly became dominant (8,9).

References

1. Zhu N., Zhang D., Wang W., et al. China Novel Coronavirus Investigating and Research Team. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med., 2020;382:727–733.
2. Hoffmann M., Kleine-Weber H., Schroeder S., et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 2020;181(2):271-280.
3. Wrapp D., Wang N., Corbett KS., et al. Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation. Science, 2020;367(6483):1260-1263.
4. Yin HS., Wen X., Paterson RG., Lamb RA., Jardetzky TS. Structure of the parainfluenza virus 5 F protein in its metastable, prefusion conformation. Nature, 2006;439(7072):38-44.
5. Amanat, F., Stadlbauer, D., Strohmeier, S., et al. A serological assay to detect SARS-CoV-2 seroconversion in humans. Nat Med., 2020;26:1033–1036.
6. Stadlbauer, D., Amanat, F., Chromikova, V., et al. SARS?CoV?2 seroconversion in humans: A detailed protocol for a serological assay, antigen production, and test setup. Current Protocols in Microbiology, 2020;57, e100.
7. Korber, B., Fischer, W., Gnanakaran, S., et al. Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus. Cell, 2020;182:1-16.
8. Tegally, H., Wilkinson, E., Giovanetti, M., et al. Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple Spike mutations in South Africa. MedRxiv, 21.12.2020;182:1-16.

NCBI and Uniprot Product Information

• UniProt Accession #: P0DTC2
• Molecular Weight: Estimated Molecular Weight: ~190 kDa