MYOF sirna

MYOF siRNA (Human)

Cat. Num. AAA8219327

• Gene Names: MYOF; FER1L3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYOF; MYOF siRNA (Human); FER1L3; KIAA1207; Myoferlin; Fer-1-like protein 3; MYOF sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYOF siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2061

• Applicable Applications for MYOF sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYOF gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYOF sirna

siRNA to inhibit MYOF expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7305053
• NCBI GeneID: 26509
• NCBI Accession #: NP_038479.1
• NCBI GenBank Nucleotide #: NM_013451.3
• UniProt Accession #: Q9NZM1; Q5VWW2; Q5VWW3; Q5VWW4; Q5VWW5; Q7Z642; Q8IWH0; Q9HBU3; Q9NZM0; Q9ULL3; Q9Y4U4; B3KQN5
• Molecular Weight: 46,695 Da
• NCBI Official Full Name: myoferlin isoform a
• NCBI Official Synonym Full Names: myoferlin
• NCBI Official Symbol: MYOF
• NCBI Official Synonym Symbols: FER1L3
• NCBI Protein Information: myoferlin
• UniProt Protein Name: Myoferlin
• Protein Family: Myosin
• UniProt Gene Name: MYOF
• UniProt Synonym Gene Names: FER1L3; KIAA1207
• UniProt Entry Name: MYOF_HUMAN

NCBI Description

Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined. [provided by RefSeq, Dec 2008]

Uniprot Description

myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: nuclear membrane; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; integral to membrane; plasma membrane; nuclear envelope; caveola; cytoplasmic vesicle

Molecular Function: protein binding; phospholipid binding

Biological Process: muscle contraction; plasma membrane repair; blood circulation; regulation of vascular endothelial growth factor receptor signaling pathway; myoblast fusion