BACE2 sirna

BACE2 siRNA (Mouse)

Cat. Num. AAA8219303

• Gene Names: Bace2; ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BACE2; BACE2 siRNA (Mouse); Beta-secretase 2; Aspartyl protease 1; ASP1; Asp 1; Beta-site amyloid precursor protein cleaving enzyme 2; Beta-site APP cleaving enzyme 2; Memapsin-1; Membrane-associated aspartic protease 1; Theta-secretase; BACE2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BACE2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 514

• Applicable Applications for BACE2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BACE2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BACE2 sirna

siRNA to inhibit BACE2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 244798416
• NCBI GeneID: 56175
• NCBI Accession #: NP_062390.3
• NCBI GenBank Nucleotide #: NM_019517.4
• UniProt Accession #: Q9JL18; Q3TNS7; Q8C5E9; Q8C793; Q9R1P7
• Molecular Weight: 55,800 Da
• NCBI Official Full Name: beta-secretase 2
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: Bace2
• NCBI Official Synonym Symbols: ARP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• NCBI Protein Information: beta-secretase 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: Bace2
• UniProt Synonym Gene Names: ASP1; Asp 1; Beta-site APP cleaving enzyme 2
• UniProt Entry Name: BACE2_MOUSE

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.45; Membrane protein, integral; Protease

Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; integral to membrane; endosome

Molecular Function: peptidase activity; hydrolase activity; aspartic-type endopeptidase activity

Biological Process: negative regulation of amyloid precursor protein biosynthetic process; membrane protein ectodomain proteolysis; proteolysis