CALCA sirna

CALCA siRNA (Mouse)

Cat. Num. AAA8219003

• Gene Names: Calca; CA; Ct; Ctn; Calc; Cgrp; CGRP1; Calc1; CGRP-1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CALCA; CALCA siRNA (Mouse); CALC; Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I; CALCA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CALCA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 128

• Applicable Applications for CALCA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CALCA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CALCA sirna

siRNA to inhibit CALCA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 76880463
• NCBI GeneID: 12310
• NCBI Accession #: NP_001029126.1
• NCBI GenBank Nucleotide #: NM_001033954.3
• UniProt Accession #: Q99JA0; P70160
• Molecular Weight: 15,141 Da
• NCBI Official Full Name: calcitonin gene-related peptide 1 isoform Cgrp preproprotein
• NCBI Official Synonym Full Names: calcitonin/calcitonin-related polypeptide, alpha
• NCBI Official Symbol: Calca
• NCBI Official Synonym Symbols: CA; Ct; Ctn; Calc; Cgrp; CGRP1; Calc1; CGRP-1
• NCBI Protein Information: calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin gene-related peptide 1
• Protein Family: Calcitonin
• UniProt Gene Name: Calca
• UniProt Synonym Gene Names: Calc; CGRP-I
• UniProt Entry Name: CALCA_MOUSE

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide (CGRP) and katacalcin. Alternative splicing of the mRNA results in multiple variants that encode either calcitonin or CGRP preproproteins. Post-translational processing of the calcitonin and CGRP propeptides results in either calcitonin and katacalcin, or CGRP, respectively. Calcitonin and katacalcin modulate calcium levels in the blood stream. CGRP can function as a vasodilator and play a role in the transmission of pain. The human homolog of CGRP was found to have antimicrobial activity. [provided by RefSeq, Mar 2015]

Uniprot Description

CALCA iso3: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; neuron projection; cell soma; cell; axon; cytoplasm; extracellular region; terminal button; intracellular; nucleus

Molecular Function: calcitonin receptor binding; hormone activity; receptor binding

Biological Process: positive regulation of cell adhesion; regulation of heart rate; adenylate cyclase activation; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure; response to pain; G-protein signaling, adenylate cyclase activating pathway; vasodilation; positive regulation of ossification; regulation of heart contraction; cellular calcium ion homeostasis; regulation of systemic arterial blood pressure by neurological process; neuropeptide signaling pathway; response to heat; negative regulation of smooth muscle contraction; regulation of blood pressure; negative regulation of ossification; feeding behavior; negative regulation of blood pressure; detection of temperature stimulus involved in sensory perception of pain; inflammatory response; negative regulation of neurological process; neuromuscular junction development; sperm capacitation