Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Goat TCF3/ITF1 Polyclonal Antibody | anti-TCF3 antibody
Goat anti-TCF3/ITF1, Biotinylated Antibody
Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]
Uniprot Description
E2A: a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.
Protein type: DNA-binding; Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: cytoplasm; nuclear chromatin; nucleoplasm; nucleus; protein complex; transcription factor complex
Molecular Function: bHLH transcription factor binding; DNA binding; mitogen-activated protein kinase kinase kinase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding
Biological Process: B cell differentiation; B cell lineage commitment; immunoglobulin V(D)J recombination; negative regulation of transcription from RNA polymerase II promoter; positive regulation of B cell proliferation; positive regulation of cell cycle; positive regulation of muscle cell differentiation; positive regulation of neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription, DNA-dependent
Research Articles on TCF3
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Product Notes
The TCF3 tcf3 (Catalog #AAA423597) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TCF3/ITF1, Biotinylated Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF3/ITF1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 75kDa band observed in lysates of cell line Daudi (calculated MW of 67.6kDa according to NP_003191.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TCF3 tcf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KAPRARTSPD EDED. It is sometimes possible for the material contained within the vial of "TCF3/ITF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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