SLC2A1 sirna

SLC2A1 siRNA (Rat)

Cat. Num. AAA8218835

• Gene Names: Slc2a1; GTG1; Gtg3; GLUTB; Glut1; RATGTG1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC2A1; SLC2A1 siRNA (Rat); GLUT-1; GLUT1; Solute carrier family 2. facilitated glucose transporter member 1; Glucose transporter type 1. erythrocyte/brain; SLC2A1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: SLC2A1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 492

• Applicable Applications for SLC2A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat SLC2A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC2A1 sirna

siRNA to inhibit SLC2A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20301952
• NCBI GeneID: 24778
• NCBI Accession #: NP_620182.1
• NCBI GenBank Nucleotide #: NM_138827.1
• UniProt Accession #: P11167
• Molecular Weight: 53,963 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 1
• NCBI Official Symbol: Slc2a1
• NCBI Official Synonym Symbols: GTG1; Gtg3; GLUTB; Glut1; RATGTG1
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: Slc2a1
• UniProt Synonym Gene Names: Glut-1; Glut1; GLUT-1
• UniProt Entry Name: GTR1_RAT

NCBI Description

transporter for glucose and other hexoses; may be involved in response to osmotic and metabolic stress [RGD, Feb 2006]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter

Cellular Component: cortical actin cytoskeleton; basolateral plasma membrane; integral to plasma membrane; integral to membrane; female pronucleus; intercellular junction; caveola; cytosol; lipid raft; membrane; cytoplasm; plasma membrane; melanosome; intracellular; midbody; vesicle

Molecular Function: D-glucose transmembrane transporter activity; identical protein binding; xenobiotic transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; sugar:hydrogen ion symporter activity; glucose transmembrane transporter activity; kinase binding

Biological Process: cellular response to glucose starvation; proton transport; xenobiotic transport; protein complex assembly; glucose transport; response to osmotic stress; transmembrane transport