ICT1 sirna

ICT1 siRNA (Human)

Cat. Num. AAA8218128

• Gene Names: ICT1; DS1; DS-1; MRP-L58
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ICT1; ICT1 siRNA (Human); DS1; Peptidyl-tRNA hydrolase ICT1 mitochondrial; 39S ribosomal protein L58 mitochondrial; MRP-L58; Digestion substraction 1; DS-1; Immature colon carcinoma transcript 1 protein; ICT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ICT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 229

• Applicable Applications for ICT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ICT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ICT1 sirna

siRNA to inhibit ICT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 737676317
• NCBI GeneID: 3396
• NCBI Accession #: NP_001290194.1
• NCBI GenBank Nucleotide #: NM_001303265.1
• UniProt Accession #: Q14197; Q53HM7; Q53Y11; B2RAD1
• Molecular Weight: 23,630 Da
• NCBI Official Full Name: peptidyl-tRNA hydrolase ICT1, mitochondrial isoform 2
• NCBI Official Synonym Full Names: immature colon carcinoma transcript 1
• NCBI Official Symbol: ICT1
• NCBI Official Synonym Symbols: DS1; DS-1; MRP-L58
• NCBI Protein Information: peptidyl-tRNA hydrolase ICT1, mitochondrial
• UniProt Protein Name: Peptidyl-tRNA hydrolase ICT1, mitochondrial
• Protein Family: 1-acylglycerol-3-phosphate O-acyltransferase
• UniProt Gene Name: ICT1
• UniProt Synonym Gene Names: DS1; MRP-L58; DS-1
• UniProt Entry Name: ICT1_HUMAN

NCBI Description

The protein encoded by this gene is a peptidyl-tRNA hydrolase and a vital component of the large mitochondrial ribosome. The encoded protein serves as a ribosome release factor for this ribosome, which translates mitochondrial genes. This protein may be responsible for degrading prematurely-terminated polypeptides and for reusing stalled ribosomes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Uniprot Description

ICT1: Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Belongs to the prokaryotic/mitochondrial release factor family. ICT1 subfamily.

Protein type: EC 3.1.1.29; Mitochondrial

Chromosomal Location of Human Ortholog: 17q25.1

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: aminoacyl-tRNA hydrolase activity; translation release factor activity, codon nonspecific

Biological Process: mitochondrial translation; organelle organization and biogenesis