ADC sirna

ADC siRNA (Human)

Cat. Num. AAA8216189

• Gene Names: AZIN2; ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADC; ADC siRNA (Human); KIAA1945; ODCP; Arginine decarboxylase; ARGDC; ODC-paralogue; ODC-p; Ornithine decarboxylase-like protein; ADC sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ADC siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 204

• Applicable Applications for ADC sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ADC gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADC sirna

siRNA to inhibit ADC expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 649572333
• NCBI GeneID: 113451
• NCBI Accession #: NP_001280491.1
• NCBI GenBank Nucleotide #: NM_001293562.1
• UniProt Accession #: Q96A70; Q5TIF4; Q5TIF5; Q5TIF6; Q8TF56; Q96L54; Q96L55; Q96L56; Q96L57; Q96MD9; B2RDU5; D3DPQ9
• Molecular Weight: 22,300 Da
• NCBI Official Full Name: antizyme inhibitor 2 isoform 1
• NCBI Official Synonym Full Names: antizyme inhibitor 2
• NCBI Official Symbol: AZIN2
• NCBI Official Synonym Symbols: ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L
• NCBI Protein Information: antizyme inhibitor 2
• UniProt Protein Name: Antizyme inhibitor 2
• Protein Family: Acetoacetate decarboxylase
• UniProt Gene Name: AZIN2
• UniProt Synonym Gene Names: ADC; KIAA1945; ODCP; AzI2; ADC; ARGDC; ODC-like protein; ODC-p
• UniProt Entry Name: AZIN2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

ADC: Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity. Belongs to the Orn/Lys/Arg decarboxylase class-II family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Mitochondrial; EC 4.1.1.19; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: transport vesicle; ER-Golgi intermediate compartment membrane; cis-Golgi network; mitochondrion; perinuclear region of cytoplasm; axon; dendrite; perikaryon; trans-Golgi network; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: protein binding; putrescine transmembrane transporter activity; ornithine decarboxylase activator activity; catalytic activity

Biological Process: positive regulation of catalytic activity; polyamine biosynthetic process; ornithine metabolic process; spermatogenesis; negative regulation of protein catabolic process; putrescine transport; polyamine metabolic process