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Western Blot (WB) (Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.)

anti-Human, Mouse MAP2 Monoclonal Antibody | anti-MAP2 antibody

Anti-MAP2 (N-term) Mouse mAb

Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry
Purity
Affinity Purified
Synonyms
MAP2; Monoclonal Antibody; Anti-MAP2 (N-term) Mouse mAb; DKFZp686I2148; Dendrite specific MAP; MAP 2; MAP dendrite specific; MAP-2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2; Mtap 2; MTAP2_HUMAN; anti-MAP2 antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1D7-B9-C8-F6
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150mM NaCl)with 0.2%sodium azide, 0.1%BSA, 50%glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
559
Applicable Applications for anti-MAP2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
Application Notes
WB: 1:1000
ICC: 1:150
Immunogen
Purified recombinant human MAP2 (N-terminus) protein fragments expressed in E Coli
Sensitivity
This antibody detects endogenous levels of MAP2 (N-terminus) and does not cross-react with related proteins.
Sequence Similarities
Contains 3 Tau/MAP repeats.
Subcellular Location
Cytoplasm >cytoskeleton

Western Blot (WB)

(Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.)

Western Blot (WB) (Western blot detection of MAP2 (N-terminus) in Mouse Brain tissue and Hela cell lysates using MAP2 (N-terminus) mouse mAb (1:1000 diluted).Predicted band size: 202KDa.Observed band size: 300KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-MAP2 (N-term) mouse mAb (dilution 1:100).)

Immunocytochemistry (ICC) (Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-MAP2 (N-term) mouse mAb (dilution 1:100).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using MAP2 (N-terminus) mouse mAb.)
Related Product Information for anti-MAP2 antibody
Entrez Summary: This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
UniPort Summary Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization.They also seem to have a stiffening effect on microtubules.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
300
NCBI Official Full Name
microtubule-associated protein 2 isoform 5
NCBI Official Synonym Full Names
microtubule associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C
NCBI Protein Information
microtubule-associated protein 2
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Synonym Gene Names
MAP-2

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: cytoplasm; microtubule associated complex; neuron projection; nucleolus

Molecular Function: microtubule binding; protein binding

Biological Process: central nervous system neuron development; dendrite morphogenesis; microtubule cytoskeleton organization and biogenesis; neurite development

Research Articles on MAP2

Similar Products

Product Notes

The MAP2 map2 (Catalog #AAA475033) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAP2 (N-term) Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC). WB: 1:1000 ICC: 1:150. Researchers should empirically determine the suitability of the MAP2 map2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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