NME1 sirna

NME1 siRNA (Rat)

Cat. Num. AAA8215596

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NME1; NME1 siRNA (Rat); Nucleoside diphosphate kinase A; NDK A; NDP kinase A; Metastasis inhibition factor NM23; Tumor metastatic process-associated protein; NME1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: NME1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 152

• Applicable Applications for NME1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat NME1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NME1 sirna

siRNA to inhibit NME1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19924089
• NCBI GeneID: 191575
• NCBI Accession #: NP_612557.1
• NCBI GenBank Nucleotide #: NM_138548.2
• UniProt Accession #: Q05982
• Molecular Weight: 17,193 Da
• NCBI Official Full Name: nucleoside diphosphate kinase A
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: Nme1
• NCBI Protein Information: nucleoside diphosphate kinase A
• UniProt Protein Name: Nucleoside diphosphate kinase A
• Protein Family: Nucleoside diphosphate kinase
• UniProt Gene Name: Nme1
• UniProt Synonym Gene Names: NDK A; NDP kinase A
• UniProt Entry Name: NDKA_RAT

NCBI Description

the A isoform of the nucleoside diphosphate kinase; plays a role in nucleotide triphosphate metabolism [RGD, Feb 2006]

Uniprot Description

NME1: an enzyme with multiple activities including histidine protein kinase, nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase and 3'-5' exonuclease activities. Plays a major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Involved in cell proliferation, differentiation and development, G protein-coupled receptor endocytosis, cytotoxic T cell and NK cell-mediated cell death, and gene expression. Required for neural development including neural patterning and cell fate determination. During granzyme A (GZMA)-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair. Three alternatively spliced human proteins have been reported.

Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Protein kinase, histidine; Kinase, nucleoside diphosphate; Tumor suppressor; Kinase, other; EC 2.7.4.6; Other group; NDK family

Cellular Component: ruffle; centrosome; mitochondrion; membrane; lamellipodium; perinuclear region of cytoplasm; intermediate filament; cytosol; nucleus; myelin sheath

Molecular Function: identical protein binding; gamma-tubulin binding; GTP binding; metal ion binding; nucleoside diphosphate kinase activity; intermediate filament binding; magnesium ion binding; protein kinase binding; protein binding; enzyme binding; deoxyribonuclease activity; ribosomal small subunit binding; ATP binding; single-stranded DNA binding

Biological Process: response to drug; integrin-mediated signaling pathway; GTP biosynthetic process; lactation; response to cAMP; negative regulation of myeloid leukocyte differentiation; UTP biosynthetic process; hippocampus development; nucleoside diphosphate phosphorylation; endocytosis; response to testosterone stimulus; DNA catabolic process; regulation of apoptosis; CTP biosynthetic process; mammary gland development; pyrimidine nucleotide metabolic process; nucleoside triphosphate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of epithelial cell proliferation; response to amine stimulus; positive regulation of DNA binding; positive regulation of keratinocyte differentiation; purine nucleotide metabolic process; negative regulation of apoptosis