A2M sirna

A2M siRNA (Rat)

Cat. Num. AAA8214960

• Gene Names: A2m; Mam; A2m1; A2mb; A2maa; A2MAC1
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: A2M; A2M siRNA (Rat); A2M1; Alpha-2-macroglobulin; Alpha-2-M; A2M sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: A2M siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1472

• Applicable Applications for A2M sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat A2M gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for A2M sirna

siRNA to inhibit A2M expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 158138551
• NCBI GeneID: 24153
• NCBI Accession #: NP_036620.2
• NCBI GenBank Nucleotide #: NM_012488.2
• UniProt Accession #: P06238; Q4FZY3
• Molecular Weight: 163,785 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2m
• NCBI Official Synonym Symbols: Mam; A2m1; A2mb; A2maa; A2MAC1
• NCBI Protein Information: alpha-2-macroglobulin
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2m
• UniProt Synonym Gene Names: A2m1; Alpha-2-M
• UniProt Entry Name: A2MG_RAT

NCBI Description

plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]

Uniprot Description

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.