PARK7 sirna

PARK7 siRNA (Rat)

Cat. Num. AAA8213743

• Gene Names: Park7; Dj1; CAP1; DJ-1; SP22
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PARK7; PARK7 siRNA (Rat); CAP1; Protein DJ-1; Contraception-associated protein 1; Protein CAP1; Fertility protein SP22; Parkinson disease protein 7 homolog; PARK7 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: PARK7 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 189

• Applicable Applications for PARK7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PARK7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PARK7 sirna

siRNA to inhibit PARK7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 471013391
• NCBI GeneID: 117287
• NCBI Accession #: NP_001264179.1
• NCBI GenBank Nucleotide #: NM_001277250.1
• UniProt Accession #: O88767
• Molecular Weight: 19,974 Da
• NCBI Official Full Name: protein deglycase DJ-1 isoform 2
• NCBI Official Synonym Full Names: parkinson protein 7
• NCBI Official Symbol: Park7
• NCBI Official Synonym Symbols: Dj1; CAP1; DJ-1; SP22
• NCBI Protein Information: protein deglycase DJ-1
• UniProt Protein Name: Protein deglycase DJ-1
• Protein Family: Protein deglycase
• UniProt Gene Name: Park7
• UniProt Synonym Gene Names: Protein CAP1
• UniProt Entry Name: PARK7_RAT

NCBI Description

displays a specific localization pattern to the anterior-ventral surface of the equatorial segment; may play a role in sperm-egg interactions and fertilization [RGD, Feb 2006]

Uniprot Description

DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.

Protein type: Transcription regulation; EC 3.4.-.-; Tumor suppressor; Nuclear receptor co-regulator

Cellular Component: PML body; mitochondrion; axon; endoplasmic reticulum; cytoplasm; plasma membrane; chromatin; cytosol; mitochondrial respiratory chain complex I; nucleus; lipid raft

Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; mercury ion binding; transcription coactivator activity; transcription factor binding; mRNA binding; peptidase activity; protein binding; copper ion binding; enzyme binding; peroxiredoxin activity; androgen receptor binding; cytokine binding; double-stranded DNA binding; glyoxalase III activity; kinase binding; superoxide dismutase copper chaperone activity; single-stranded DNA binding; receptor binding

Biological Process: synaptic transmission, dopaminergic; negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; regulation of mitochondrial membrane potential; negative regulation of protein amino acid phosphorylation; single fertilization; regulation of neuron apoptosis; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; response to drug; mitochondrion organization and biogenesis; protein stabilization; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of protein sumoylation; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; fertilization; response to hydrogen peroxide; detoxification of mercury ion; regulation of inflammatory response; hydrogen peroxide metabolic process; negative regulation of ubiquitin-protein ligase activity; autophagy; negative regulation of protein kinase activity; lactate biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein ubiquitination; positive regulation of transcription factor activity; response to oxidative stress; negative regulation of apoptosis