SFRP2 sirna

SFRP2 siRNA (Mouse)

Cat. Num. AAA8213522

• Gene Names: Sfrp2; Sdf5; AI851596
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SFRP2; SFRP2 siRNA (Mouse); SARP1; SDF5; Secreted frizzled-related protein 2; sFRP-2; Protein SDF5; Secreted apoptosis-related protein 1; SARP-1; SFRP2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SFRP2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 295

• Applicable Applications for SFRP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SFRP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SFRP2 sirna

siRNA to inhibit SFRP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6677895
• NCBI GeneID: 20319
• NCBI Accession #: NP_033170.1
• NCBI GenBank Nucleotide #: NM_009144.2
• UniProt Accession #: P97299; O08862; O35297
• Molecular Weight: 33,469 Da
• NCBI Official Full Name: secreted frizzled-related protein 2
• NCBI Official Synonym Full Names: secreted frizzled-related protein 2
• NCBI Official Symbol: Sfrp2
• NCBI Official Synonym Symbols: Sdf5; AI851596
• NCBI Protein Information: secreted frizzled-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 2
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp2
• UniProt Synonym Gene Names: Sarp1; Sdf5; sFRP-2; SARP-1
• UniProt Entry Name: SFRP2_MOUSE

Uniprot Description

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: Wnt-protein binding; protein binding; receptor agonist activity

Biological Process: neural tube development; convergent extension involved in axis elongation; collagen fibril organization; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; apoptosis; multicellular organismal development; negative regulation of caspase activity; signal transduction; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; regulation of apoptosis; negative regulation of cell proliferation; negative regulation of mesodermal cell fate specification; positive regulation of cell proliferation; cell differentiation; negative regulation of cell migration; negative regulation of epithelial cell proliferation; response to drug; negative regulation of JNK activity; negative regulation of peptidyl-tyrosine phosphorylation; somitogenesis; Wnt receptor signaling pathway; male gonad development; regulation of Wnt receptor signaling pathway; digestive tract morphogenesis; chondrocyte development; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; patterning of blood vessels; positive regulation of osteoblast differentiation; development of primary male sexual characteristics; positive regulation of angiogenesis; cartilage development; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent