MAP3K3 sirna

MAP3K3 siRNA (Human)

Cat. Num. AAA8212788

• Gene Names: MAP3K3; MEKK3; MAPKKK3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MAP3K3; MAP3K3 siRNA (Human); MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3; MAP3K3 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MAP3K3 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 626

• Applicable Applications for MAP3K3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MAP3K3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MAP3K3 sirna

siRNA to inhibit MAP3K3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 42794765
• NCBI GeneID: 4215
• NCBI Accession #: NP_002392.2
• NCBI GenBank Nucleotide #: NM_002401.3
• UniProt Accession #: Q99759; Q5BKZ6; Q8N3I9; B2RCW2; D3DU15
• Molecular Weight: 74,089 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: MAP3K3
• NCBI Official Synonym Symbols: MEKK3; MAPKKK3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: MAP3K3
• UniProt Synonym Gene Names: MAPKKK3; MEKK3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_HUMAN

NCBI Description

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: EC 2.7.11.25; Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: intracellular; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade