Western Blot (WB)
(Western Blot analysis of mouse cerebellum tissue lysates (35ug/lane) using MBS648219 . ATG1 (arrow) was detected using MBS648219.)
Rabbit anti-Human, Mouse ATG1, Denatured Polyclonal Antibody
ATG1, Denatured (ULK1, KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
PurifiedPurified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
ATG1; Denatured; Polyclonal Antibody; Denatured (ULK1; KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1); Anti -ATG1; anti-ATG1; Denatured antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ATG1, Denatured antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:50-100
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
denatured ATG1 antibody is generated from rabbits immunized with a denatured human ATG1 recombinant protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of mouse cerebellum tissue lysates (35ug/lane) using MBS648219 . ATG1 (arrow) was detected using MBS648219.)
Western Blot (WB)
(Western Blot analysis of mouse cerebellum tissue lysates (35ug/lane) using MBS648219 . ATG1 (arrow) was detected using MBS648219.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human skeletal muscle reacted using MBS648219, followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of MBS648219 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human skeletal muscle reacted using MBS648219, followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of MBS648219 for immunohistochemistry.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of k562 cells using MBS648219 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow Cytometric analysis of k562 cells using MBS648219 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-ATG1, Denatured antibody
ULK1 is involved in autophagy. It is required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. It is involved in axon growth (By similarity) and plays an essential role in neurite extension of cerebellar granule cells (By similarity).
Product Categories/Family for anti-ATG1, Denatured antibody
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Product Notes
The ATG1, Denatured (Catalog #AAA648219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG1, Denatured (ULK1, KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG1, Denatured can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the ATG1, Denatured for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG1, Denatured, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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